Anethole, a Medicinal Plant Compound, Decreases the Production of Pro-Inflammatory TNF-α and IL-1β in a Rat Model of LPS-Induced Periodontitis

Moradi, Janet; Abbasipour, Fatemeh; Zaringhalam, Jalal; Ziaee, Narges; Khodadoustan, Amin; Janahmadi, Mahyar

doi:10.22037/ijpr.2014.1598

Anethole, a Medicinal Plant Compound, Decreases the Production of Pro-Inflammatory TNF-α and IL-1β in a Rat Model of LPS-Induced Periodontitis

Document Type : Research article

Authors

¹ Department of Periodontics, Dental School, Hamadan University of Medical Sciences

² Dep. of Physiology, Medical School, Shahid Beheshti University of Medical Sciences

³ Department of Periodontics, Dental School, Hamadan University of Medical Sciences, Hamadan, Iran

⁴ Private practice in periodontics and implant surgery

⁵ Neurophysiology Research Centre and Dept. of Physiology , Medical School, Shahid Beheshti University of Medical Sciences

10.22037/ijpr.2014.1598

Abstract

Periodontitis (PD) is known to be one of most prevalent worldwide chronic inflammatory diseases. There are several treatments including antibiotics for PD; however, since drug resistance is an increasing problem, new drugs particularly derived from plants with fewer side effects are required. The effects of trans-anethole on IL-1 β and TNF-α level in a rat model of PD were investigated and compared to ketoprofen.
Eschericia coli lipopolysaccharide (LPS, 30 µg) was injected bilaterally into the palatal gingiva (3µl/site) between the upper first and second molars every two days for 10 days in anesthetized rats. Administration of either trans-anethole (10 or 50mg/kg, i.p.) or ketoprofen (10 mg/kg, i.p.) was started 20 minute before LPS injection and continued for 10 days. Then, IL-1β and TNF-α levels were measured in blood samples by ELISA at day 0 (control) and at day 10.
Anethole at both concentrations significantly suppressed IL-1β and TNF-α production when compared to LPS-treated rats. The suppressive effects of anethole on LPS-induced pro-inflammatory cytokines were almost similar as seen with ketoprofen.
In conclusion, the present results suggest that anethole may have a potent inhibitory effect on PD through suppression of pro-inflammatory molecules; therefore it could be a novel therapeutic strategy for PD.

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