Anethole, a Medicinal Plant Compound, Decreases the Production of Pro-Inflammatory TNF-α and IL-1β in a Rat Model of LPS-Induced Periodontitis

Document Type : Research article

Authors

1 Department of Periodontics, Dental School, Hamadan University of Medical Sciences

2 Dep. of Physiology, Medical School, Shahid Beheshti University of Medical Sciences

3 Department of Periodontics, Dental School, Hamadan University of Medical Sciences, Hamadan, Iran

4 Private practice in periodontics and implant surgery

5 Neurophysiology Research Centre and Dept. of Physiology , Medical School, Shahid Beheshti University of Medical Sciences

Abstract

Periodontitis (PD) is known to be one of most prevalent worldwide chronic inflammatory diseases. There are several treatments including antibiotics for PD; however, since drug resistance is an increasing problem, new drugs particularly derived from plants with fewer side effects are required. The effects of trans-anethole on IL-1 β and TNF-α level in a rat model of PD were investigated and compared to ketoprofen.
Eschericia coli lipopolysaccharide (LPS, 30 µg) was injected bilaterally into the palatal gingiva (3µl/site) between the upper first and second molars every two days for 10 days in anesthetized rats. Administration of either trans-anethole (10 or 50mg/kg, i.p.) or ketoprofen (10 mg/kg, i.p.) was started 20 minute before LPS injection and continued for 10 days. Then, IL-1β and TNF-α levels were measured in blood samples by ELISA at day 0 (control) and at day 10.
Anethole at both concentrations significantly suppressed IL-1β and TNF-α production when compared to LPS-treated rats. The suppressive effects of anethole on LPS-induced pro-inflammatory cytokines were almost similar as seen with ketoprofen.
In conclusion, the present results suggest that anethole may have a potent inhibitory effect on PD through suppression of pro-inflammatory molecules; therefore it could be a novel therapeutic strategy for PD.

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