Document Type : Research article
College of Resources and Environment, Southwest University, Chongqing, 400716, P.R. China
Chongqing Academy of Chinese Materia Medica, Chongqing 400065, P.R.China
College of Life Sciences, Chongqing Normal University, Chongqing 401331, P.R.China.
College of Bioengineering, Chongqing University, Chongqing 400044, PR China.
The aim of this paper is to develop a rapid, and highly sensitive quantitative HPLC fingerprint method with multiple indicators by using the Compound Chinese Medicine Wuwei Changyanning granule and 5 herbs in the prescription. The quantitative fingerprint chromatogram with multiple indicators was investigated. ⅰ)6 compositions included rutin, gallic acid, chlorogenic acid, atractylenolideⅠ, pachymic acid and apigenin, which originated from 5 herbs respectively, were selected as quantitative compositions, and their contents were determined using HPLC from 11 batches granules and the corresponding 5 medicinal materials. ⅱ) The precision, stability and repeatability of fingerprinting were investigated. In addition, common peaks number, the percentage of non-common peaks and similarity were also studied. Among them, 21 common peaks in the granule could find the source of peaks from the 5 herbs, among of 10 peaks from Niuerfeng, 9 peaks from Laliao, 3 peaks from Baishu,3 peaks from Fuling and 5peaks from Guanghuoxiang. The results showed that the identification method of fingerprinting was reliable.