Optimization of Topography and Surface Properties of Polyacrylonitrile-Based Electrospun Scaffolds via Nonoclay Concentrations and its Effect on Osteogenic Differentiation of Human Mesenchymal Stem Cells

Document Type : Research article

Authors

1 Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

2 Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Science, Tehran, Iran.

3 Department of Tissue Engineering and Regenerative Medicine, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

4 Laser Application in Medical Sciences Research Center, Shaid Beheshti University of Medical Sciences, Tehran, Iran.

5 Hearing Disorders Research Center, Loghman Hakim Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

6 Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.

10.22037/ijpr.2021.115119.15208

Abstract

Nowadays, mesenchymal stem cells (MSCs) are the most widely used cell sources for bone regenerative medicine. Electrospun polyacrylonitrile (PAN)-based scaffolds play an important role in bone tissue engineering due to their good mechanical properties, which could be enhanced by the presence of nanoparticles such as nanoclay. This study evaluated the in-vitro effect of different concentrations of nanoclay in surface characteristic properties of PAN-based electrospun nanofiber scaffolds and the osteogenic differentiation ability of adipose-derived mesenchymal stem cells (AD-MSCs). After electrospinning nanofibers, their structure were assessed through some characterization tests. Then AD-MSCs isolation and characterization were done, and the cell attachment and the biocompatibility were determined. Finally, osteogenic differentiation-related markers, genes, and proteins were studied. Clay-PAN25% electrospun nanofiber scaffold could support attachment, proliferation, and osteogenic differentiation of AD-MSCs better than other groups. Also, nanoclay could enhance the properties of PAN-based scaffolds, such as fiber diameter, topography, surface charge, hydrophilicity, roughness, and degradation, as well as osteogenic differentiation of cells. As a result, Clay-PAN25% with the highest concentration of nanoclay was found as a promising biodegradable and cost-effective scaffold for osteogenic differentiation of AD-MSCs.

Graphical Abstract

Optimization of Topography and Surface Properties of Polyacrylonitrile-Based Electrospun Scaffolds via Nonoclay Concentrations and its Effect on Osteogenic Differentiation of Human Mesenchymal Stem Cells

Keywords


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