Detection of Oxytetracycline Residue in Infant Formula by High-Performance Liquid Chromatography (HPLC)

Document Type: Research article

Authors

1 Food and Drug Laboratory Research center, Ministry of Health and Medical Education, Tehran, Iran. Food and Drug Control Laboratories, Ministry of Health and Medical Education, Tehran, Iran.

2 Departments of Toxicology and Pharmacology, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran. Departmants of Toxicology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

3 ood and Drug Laboratory Research center, Ministry of Health and Medical Education, Tehran, Iran. Food and Drug Control Laboratories, Ministry of Health and Medical Education, Tehran, Iran.

Abstract

Determination of drug residues in food is of great importance due to their toxicity. Long-term exposure with low level of drug residues could be important, especially in children. Based on document study, oxytetracycline (OTC) is a prophylaxis and treatment agent for great number of diseases and possesses a broad spectrum activity against many pathogenic organisms and can be toxic or cause allergic reactions in some hypersensitive individual’s if the residues of drug exist in the infant formula. The previous studies show that using high-performance liquid chromatography (HPLC) is useful for OTC detection in milk. Therefore, we decided to measure OTC in infant formula. The purpose of this study was to investigate residual OTC in consuming infant formula using HPLC. The limit of quantification (LOQ) was 51 ng/mL. This result showed that the samples had no residues of OTC in infant formula from different companies.

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