1Bioengineering College of Chongqing University, Chongqing, 400044, P.R.China. Life Science College of Guizhou Normal University, Guiyang, Guizhou, 550001, P.R.China
2Drug Screening Lab, Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Science, 550001, P.R.China
3Bioengineering College of Chongqing University, Chongqing, 400044, P.R.China
4Guizhou Provincial Laboratory for Mountainous Environment (GLE), Guizhou Normal University, Guiyang, Guizhou 550001, P.R.China.
Abstract
The chemical composition of the essential oils of flower at the pre-flowering, full-flowering and post-flowering stage of A. annua was analyzed by GC and GC/MS and sixty-two components were identified. The main compounds in the pre-flowering oil were β-myrcene (37.71%), 1, 8-cineole (16.11%) and camphor (14.97%). The full-flowering oil contained predominantly caryophyllene (19.4%), germacrene D (18.1%), camphor (15.84%), 1, 8-cineole (10.6%) and (Z)-β-farnesene (9.43%). The major constituents identified in the post-flowering oil were camphor (16.62%), caryophyllene (16.27%), β-caryophyllene oxide (15.84%), β-farnesene (9.05%) and (-)-spathulenol (7.21%).
The variety of anti-AChE activity of flower oil of A. annua at three flowering stage might be a result of the variety of the content and interaction of those terpenoids with anti-AChE activity. The greatest acetylcholinesterase inhibitory activity (IC50 = 0.13 ± 0.02 mg mL-1) was exhibited by the essential oil of flower of A. annua at post-flowering stage.
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