Increased plasma cholesterol is known to be a major risk related to the development of
coronary artery disease. The main purpose of this study was to determine possible effects
of the ethanolic extract of aerial parts of Peucedanum pastinacifolium on the serum lipids
in hypercholesterolemic rats. Experimental hypercholesterolemia was induced by feeding rats
???? ?????????????????????????????????????????? ?????????? ???????? ?????? ???????????? ?????????????????????????????????????????? ?????????? ?????????? ???????????????????? ?????????? ????????
groups. One group did not receive treatments and served as a control hypercholesterolemic
group. The other four groups were fed the cholesterol-enriched diet in conjunction with
125, 250, 500 mg extract per kg of the body weight along with 20 mg atrovaststin per kg
body weight, in a daily oral dose. A normal group of rats fed with a plain chow diet was also
included in the study. After oral administration for 8 weeks, P. pastinacifolium extract produced
levels and atherogenic indices in hypercholesterolemic groups (P < 0.05). P. pastinacifolium
extract had no effects on serum high-density lipoprotein cholesterol levels in these groups. It is
concluded that the extract of aerial parts of P. pastinacifolium exhibits lipid lowering activity in
hypercholesterolemic rats. However, further investigation would be necessary to evaluate the
mechanism of action of the extract.
Acute and Subchronic Toxicity?of Teucrium polium Total Extract in Rats
Iranian Journal of Pharmaceutical Research
(2009), 8 (4): 301-306
Received: August 2008
Accepted: January 2009
Copyright ? 2009 by School of Pharmacy Shaheed Beheshti University of Medical Sciences and Health Services
Lipid Lowering Effect of
Ethanolic Extract of Aerial Parts
of Peucedanum pastinacifolium Boiss. and Hausskn.
in Hypercholesterolemic Rats
Ahmad Movahediana*, S.Ebrahim Sajjadib and
aDepartment of Clinical Biochemistry, Isfahan
Pharmaceutical Sciences Research Center, School of Pharmacy and
Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan,
Iran. bDepartment of Pharmacognosy, School of Pharmacy and Pharmaceutical
Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
Increased plasma cholesterol is known to be a major risk related to the
development of coronary artery disease. The main purpose of this study was to
determine possible effects of the ethanolic extract of aerial parts of
Peucedanum pastinacifolium on the serum lipids in hypercholesterolemic rats.
Experimental hypercholesterolemia was induced by feeding rats a
cholesterol-enriched diet for 56 days. Hypercholesterolemic rats were classified
into five groups. One group did not receive treatments and served as a control
hypercholesterolemic group. The other four groups were fed the
cholesterol-enriched diet in conjunction with 125, 250, 500 mg extract per kg of
the body weight along with 20 mg atrovaststin per kg body weight, in a daily
oral dose. A normal group of rats fed with a plain chow diet was also included
in the study. After oral administration for 8 weeks, P. pastinacifolium extract
produced significant decrease on serum total cholesterol, low-density
lipoprotein cholesterol, triglyceride levels and atherogenic indices in
hypercholesterolemic groups (P < 0.05). P. pastinacifolium extract had no
effects on serum high-density lipoprotein cholesterol levels in these groups. It
is concluded that the extract of aerial parts of P. pastinacifolium exhibits
lipid lowering activity in hypercholesterolemic rats. However, further
investigation would be necessary to evaluate the mechanism of action of the
Cardiovascular diseases are one of the leading causes of death in the world.
Atherosclerosis plays a major role in the development of myocardial infarction
and stroke (1). Elevated plasma cholesterol, especially in LDL, is known to be a
major risk related to the development of coronary artery disease or
arteriosclerosis (2). Approximately 15% of middle-aged Americans have primary
hypercholesterolemia, that is, plasma cholesterol levels in excess of 250 mg/dL.
The risk for coronary heart disease in these patients is at least twice that of
patients with a baseline level of 200 mg/dL (3).
Several publications coming from different laboratories included recommendations
for a reduction in cholesterol consumption as a means of preventing heart
disease. At present, dyslipidemia is most commonly treated with lipid-altering
pharmacological therapies. However, safety concerns regarding the use of these
agents have prompted the need for safe and efficacious nonpharmacological
lipid-altering interventions (4). As such, the effects of dietary components on
plasma lipid metabolism have recently received considerable attention, thereby
highlighting the importance of naturally occurring compounds as cholesterol
Many herbal medicinal products have potential hypocholesterolemic activity and
encouraging safety profiles, such as Dracocephalum kotschyi (5), Allium porrum
(6), Purslane (7), Eclipta prostrata (8), Scoparia dulcis (9),
foenum-graecum, and red yeast rice (10). However, only a limited amount of
clinical research exists to support their efficacy.
Peucedanum pastinacifolium Boiss. and Hausskn. is a plant from Apiaceae family
(11). The plant have been used by local inhabitants in west of Iran, as an
antihyperlipidemic vegetable. Chemical components of P. pastinacifolium are
unknown, and there is no evidence for its pharmacological activity but there are
some studies on other Peucedanum spices which demonstrates antibacterial (12,
13), antiplatelet (14) and antimutagenic (15) activities. Phytochemical studies
on Peucedanum species led to the isolation of coumarins (16, 17) and essential
oil (18, 19). In this study we report the effect of P. pastinacifolium aerial
parts extract on serum lipids levels to evaluate hypolipidemic activity of the
Plant material and extraction
Aerial parts of P. pastinacifolium at full flowering stage were collected from
Isfahan province in May 2005 at an altitude of 1530 m. The plant identity as P. pastinacifolium was confirmed by the Botany Department of Isfahan University. A
voucher specimen of the plant was deposited in the Herbarium of the Faculty of
Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences,
Isfahan, Iran (No. 1124). The air-dried plant material were roughly cut and
ground to the powder. The plant material was macerated with EtOH-H2O (7:3) for
24 h. The hydroalcholic extract was evaporated in a rotating evaporator under
reduced pressure until dryness.
Diet and treatment
Six groups (n=36) of male wistar rats (obtained from the central animal house of
the Tehran Pasteur Institute, Tehran, Iran) weighting 200?20 g at the beginning
of the study were used. Rats were housed identically in an air-conditioned room
under a 12 h light-dark cycle. The control group was fed a standard diet (normal
control). Group 1 was fed a standard diet enriched with 1% cholesterol and 0.5%
cholic acid (hypercholesterolemic control). Groups 2-5 receiving
cholesterol-enriched diet plus 125, 250, 500 mg extract per kg of the body
weight along with 20 mg atrovaststin per kg body weight, respectively (treated
groups), in a daily oral dose. The experiment lasted eight weeks. All of the
rats were initially fed a standard laboratory diet for at least 7 days after
delivery to our laboratory for adaptation. Tap water was freely available. Food
intake was monitored daily for all control and treated groups. The different
doses of plant extract and atorvastatin were suspended in water and given orally
Measurement of body weight and biochemical parameters
The body weight (BW) of each rat was determined before starting the treatment
and each week afterward. Blood samples were collected from the jugular vein;
serum was separated by centrifugation (2000 g, 20 min, 4?C) and used for
biochemical analysis. Serum concentrations of cholesterol, triglyceride, LDL-C
and HDL-C were determined with commercially available enzyme kits (Pars Azmoon,
Tehran, Iran). The atherogenic index (total cholesterol −HDL-C/ HDL-C) was also
calculated. At the end of the treatment, the heart and liver were weighed and
the organ/ BW ratio was calculated and expressed as a percentage.
All values used in analysis are presented as mean ? SD for 6 rats in each group
of experiments. Comparisons among the different groups were performed by one-way
analysis of variance (ANOVA), followed by Tukey?s multiple comparisons test and
differences were considered significant when P <0.05.
All groups exhibited an increase in body weight through the treatment without
significant differences among them (Table 1). There were no differences in the
heart/BW ratio. However the liver/ BW ratio decreased in all of the treated
groups compared with the hypercholesterolemic group (Table 1).
The serum concentrations of total cholesterol, triglyceride and lipoproteins
(HDL-C and LDL-C) were increased in hypercholesterolemic group with respect to
the normal control group at the end of the treatment (Table 2).
Serum total cholesterol and triglyceride decreased significantly in all groups
treated with P. pastinacifolium extract but no significant differences were
observed among them (Table 3). Changes in distribution of cholesterol in HDL or
LDL were found, i.e., LDL-C decreased significantly with respect to the
hypercholesterolemic group in all groups treated with P. pastinacifolium extract
and atorvastatin. However there were no significant differences among the groups
(Table 3). Although a decrease in HDL-C was found in all treated groups in
comparison with hypercholesterolemic group, this reduction was not significant
(Table 3). Finally atherogenic index in all groups treated with P. pastinacifolium extract was decreased significantly compared with
hypercholesterolemic group since no significant differences were found between
the treated groups and control group (Table 4).
The importance of plasma levels of cholesterol and lipoproteins in the
pathogenesis of atherosclerosis has been noted by a number of studies. The
relationship between dietary cholesterol and coronary heart disease (CHD) has
been a topic of intense research, and considerable debate of the 20th century
(20). The plasma cholesterol response to dietary cholesterol is highly variable
across and within animal species, since rats exhibit little change in plasma
total cholesterol even with high doses of dietary cholesterol (20).
Our results showed that serum total cholesterol and lipoprotein cholesterol
levels in rats were increased in the hypercholesterolemic group at the end of
the 56-day study (Table 2). It was obvious in our study that the elevation in
serum total cholesterol mainly originated from the remarkable increase in LDL-C
rather than HDL-C. Various studies indicated that rats fed with a
cholesterol-rich diet do not develop cholesterolemia, instead, cholesterol must
be fed in combination with cholate. Diets supplemented with cholic acid
increased cholesterol absorption (21, 22).
Because of folkloric use of P. pastinacifolium as a lipid lowering vegetable in
west of Iran, we investigated the possible beneficial effects of the extract on
the serum lipids levels in diet induced hypercholesterolemic rats. With regard
to the effect of dietary supplements, our results showed that oral
administration of P. pastinacifolium extract improved the hyperlipidemia induced
by cholesterol-enriched diet in treated rats. As shown in the present work, oral
administration of P. pastinacifolium extract for 8 weeks resulted in a
significant reduction of serum total cholesterol, LDL-C and TG levels without
any significant changes in HDL-C at doses of 125, 250, and 500 mg/kg BW in
comparison with the hypercholesterolemic control group (Table 3). However, there
is no significant differences in serum lipid parameters in the all groups
treated with different doses of the extract. Therefore, it can be concluded from
our results that the effect of P. pastinacifolium extract was not dose dependent
at selected doses (Table 3).
To the best of our knowledge, there is no phytochemical study on
P. pastinacifolium in the literature, but presence of coumarinic compounds in other
Peucedanum species (16, 17) led us to the probable biological activity of this
group of secondary metabolites. It is proved that some furanocoumarines such as
khellactons can inhibit lipolysis induced by adrenalin which can finally affect
the lipid metabolism and reduction of lipoprotein levels (23). Laserpitin is
another beneficial coumarin compound found in Angelica keiskei that can change
lipoproteins metabolism and has beneficial effect on hyperlipidemia. This
compound produces increase in serum HDL level and decreases in the hepatic
triglyceride content (24). Another interesting study indicate that scoparone
(6,7-dimethoxycoumarin), extracted from Chinese herb (Artemisia scoparia), has
an antiatherogenic action in hyperlipideamic diabetic rabbits (25). Potential
hypocholesterolemic effects of coumarins and presence of coumarins in other
Peucedanum species indicate that coumarins are probably the active constituents
of the plant. However, further investigation are necessary to prove this
postulation and to evaluate the mechanism of action of the extract. Another
possible mechanism by which P. pastinacifolium exerted its cholesterol lowering
effect is a decrease in cholesterol absorption, by binding with bile acids
within the intestine and increasing fecal bile acids excretion. Another
mechanism related to hypolipidemic effect of Apiaceae family is hypoglycemic
effect and reduction in blood glucose. Continues supplementation of Cumminumcyminum for six weeks prevents elevation of plasma and tissue lipids secondary
to the diabetic rats. This hypolipidemic effect of C. cyminum can be explained
as result of direct reduction in the blood glucose concentration (26).
Rats treated with P. pastinacifolium extraxt also showed a decrease in the
atherogenic index compared with hypercholesterolemic control group, which is
generally believed to be beneficial since the HDL level inversely correlated
with CHD and reduction in this ratio is considered as an anti atherosclerotic
factor. This is an interesting result, as elevated concentration of serum
cholesterol (especially LDL-C) is one of the risk factors in development of
In conclusion, the current results indicate that P. pastinacifolium extract
exerts a hypocholesterolemic effect in cholesterol-fed rats and suggest a
rational basis for folk and traditional uses of this herb in Iran.
The authors wish to thank Dr. Iraj Mehregan for identifying of plant material.
This work was supported by Research Council of the Isfahan University of Medical
Sciences, Isfahan, Iran (research project No. 83445).
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