In vivo antiplatelet aggregatory activity of Aloe vera juice on mice cerebral micro vessels

Authors

Abstract

Aloe vera plant (A. vulgari and A. barbadensis) has being used traditionally as a medicinal plant for centuries. Gel from the inner central zone of the leaves and latex from pericyclic cells are used for medicinal purposes. The present study was conducted to elucidate the beneficial effects of Aloe vera juice on the platelet aggregation in cerebral microvessels of mice.
Male mice were injected with Saline (control) or Aloe vera juice (0.2 ml/30 g b.w.) one hour before the experiment. Animals were anaesthetized and trachea was intubated. Craniotomy was performed and a window was opened on the left side of the skull. Layer of dura was removed. Brain surface microvessels were exposed and animal was placed on the microscope stage. Microscope was connected to a monitor and VCR to record all events. Exposed brain surface was continuously irrigated with ACSF solution. After the body temperature was maintained at 37?C, Sodium fluorescein (2%, 0.1 ml/10 g) was injected i.v. through tail injection. After 30 seconds, high intensity mercury light was switched on to induce platelet aggregation photochemically. Appearance of first platelet aggregation and total blood flow stop were timed in seconds. Data was statistically analyzed using Mann-Whitney test.
Our results show that in the animals treated with Aloe vera juice, venule as well as arteriole platelet aggregation timings were significantly delayed (p<0.05) in comparison to the controls. [ (Control: venule 1st aggregation 25.40±1.14, flow stop 173.80±19.9; Arteriole: 1st aggregation 56.00±8.51, flow stop 140.40±8.93; Aloe vera: venule 1st aggregation 28.50±1.97, flow stop 209.67±16.11 Arteriole: 1st aggregation 103.50±7.31, flow stop 166.17±19.63)]. Data shows the beneficial influence of Aloe vera by delaying thrombus formation in the cerebral microvessels, in vivo.
This study was performed in vivo to examine the antiplatelet aggregatory effects of Aloe vera juice, a traditionally used medicinal plant. We find that Aloe vera juice is beneficial in delaying the platelet formation in the blood vessels of the brain. The delay in thrombus formation may be attributed to the presence of superior antioxidants present in the Aloe vera juice by protecting cells in the body against destruction by free radicals thus reducing the risk of arteriosclerosis.

Iranian Journal of Pharmaceutical Research (2004): Supplement 2

Iranian Journal of Pharmaceutical Research (2004): Supplement 2: 70-70
Poster Presentations
/Biological Effect of Medicinal Plants

2nd International Congress on Traditional Medicine and Materia Medica
October 4-7, 2004, Tehran, Iran

209

In vivo antiplatelet aggregatory activity of Aloe vera juice on mice cerebral micro vessels

Singh S., Fahim M.A.

Department of Physiology, Faculty of Medicine and Health Sciences, U.A.E. University, Al Ain, United Arab Emirates

Aloe vera plant (A. vulgari and A. barbadensis) has being used traditionally as a medicinal plant for centuries. Gel from the inner central zone of the leaves and latex from pericyclic cells are used for medicinal purposes. The present study was conducted to elucidate the beneficial effects of Aloe vera juice on the platelet aggregation in cerebral microvessels of mice.

Male mice were injected with Saline (control) or Aloe vera juice (0.2 ml/30 g b.w.) one hour before the experiment. Animals were anaesthetized and trachea was intubated. Craniotomy was performed and a window was opened on the left side of the skull. Layer of dura was removed. Brain surface microvessels were exposed and animal was placed on the microscope stage. Microscope was connected to a monitor and VCR to record all events. Exposed brain surface was continuously irrigated with ACSF solution. After the body temperature was maintained at 37ºC, Sodium fluorescein (2%, 0.1 ml/10 g) was injected i.v. through tail injection. After 30 seconds, high intensity mercury light was switched on to induce platelet aggregation photochemically. Appearance of first platelet aggregation and total blood flow stop were timed in seconds. Data was statistically analyzed using Mann-Whitney test.

Our results show that in the animals treated with Aloe vera juice, venule as well as arteriole platelet aggregation timings were significantly delayed (p<0.05) in comparison to the controls. [ (Control: venule 1st aggregation 25.40±1.14, flow stop 173.80±19.9; Arteriole: 1st aggregation 56.00±8.51, flow stop 140.40±8.93; Aloe vera: venule 1st aggregation 28.50±1.97, flow stop 209.67±16.11 Arteriole: 1st aggregation 103.50±7.31, flow stop 166.17±19.63)]. Data shows the beneficial influence of Aloe vera by delaying thrombus formation in the cerebral microvessels, in vivo.

This study was performed in vivo to examine the antiplatelet aggregatory effects of Aloe vera juice, a traditionally used medicinal plant. We find that Aloe vera juice is beneficial in delaying the platelet formation in the blood vessels of the brain. The delay in thrombus formation may be attributed to the presence of superior antioxidants present in the Aloe vera juice by protecting cells in the body against destruction by free radicals thus reducing the risk of arteriosclerosis.

Presenting Author: Singh, S. sarabjits@uaeu.ac.ae