Rahimi, R., Amirghofran, Z., Mohagheghzadeh, A. (2010). Cytotoxic activity of in vitro cultures of Linum spp.. Iranian Journal of Pharmaceutical Research, Volume 3(Supplement 2), 70-70.
R Rahimi; Z Amirghofran; A Mohagheghzadeh. "Cytotoxic activity of in vitro cultures of Linum spp.". Iranian Journal of Pharmaceutical Research, Volume 3, Supplement 2, 2010, 70-70.
Rahimi, R., Amirghofran, Z., Mohagheghzadeh, A. (2010). 'Cytotoxic activity of in vitro cultures of Linum spp.', Iranian Journal of Pharmaceutical Research, Volume 3(Supplement 2), pp. 70-70.
Rahimi, R., Amirghofran, Z., Mohagheghzadeh, A. Cytotoxic activity of in vitro cultures of Linum spp.. Iranian Journal of Pharmaceutical Research, 2010; Volume 3(Supplement 2): 70-70.
Cytotoxic activity of in vitro cultures of Linum spp.
There are some reports on the lignan constituents of Linum spp. In this study, we investigated the cyototoxicity of Linum spp. in vitro cultures for the first time. If they show significant cytotoxic activity, they will be valuable candidates for isolation of cytotoxic compounds in large scale from them by tissue culture technology.
In our study, we chose normal root, hairy root and calli cultures of Linum species. We investigated their cytotoxic activity on 5 human cell lines, K562 (chronic myeloid leukemia), Jurkat (T cell leukemia), Hela (cervix adenocarcinoma), A549 (lung carcinoma) and Fen (bladder carcinoma) by MTT assay.
Among studied cultures, hairy root culture of L. album showed the best cyototxic activity on all cell lines. Callus culture of L. persicum and L. glaucom had also significant cytotoxic activity on all cell lines. Normal root culture of L. mucronatum showed weak cytotoxic activity on Jurkat and significant activity on other cell lines. Hairy root culture of L. nodiflorum had no cytotoxic activity on Jurkat but significant cytotoxicity on other cell lines. Hairy root culture of L. catharticum showed no cytotoxicity on Jurkat, weak cytotoxicity on K562 and significant cytotoxic activity on other cell line.
The cyototoxic activity of Linum cultures investigated here could be due to lignan compounds. Therefore, Linum cultures appear as valuable candidates for isolation of cytotoxic compounds in large scales.
Full Text
Iranian Journal of Pharmaceutical Research (2004): Supplement 2
Iranian Journal of Pharmaceutical Research (2004): Supplement 2:70-70
Poster Presentations/Biological Effect of
Medicinal Plants
2nd International Congress on Traditional Medicine and Materia Medica October 4-7, 2004, Tehran, Iran
208
Cytotoxic activity of
in vitro cultures of Linum spp.
Rahimi R.1,
Amirghofran Z.2, Mohagheghzadeh A.1
1Department
of Pharmacognosy, Faculty of Pharmacy; 2Department of Immunology,
Faculty of Medicine, Shiraz University of Medical Sciences Shiraz, I.R, Iran
There are some reports on the lignan constituents of Linum spp. In this
study, we investigated the cyototoxicity of Linum spp. in vitro cultures
for the first time. If they show significant cytotoxic activity, they will be
valuable candidates for isolation of cytotoxic compounds in large scale from
them by tissue culture technology.
In
our study, we chose normal root, hairy root and calli cultures of Linum
species. We investigated their cytotoxic activity on 5 human cell lines, K562
(chronic myeloid leukemia), Jurkat (T cell leukemia), Hela (cervix
adenocarcinoma), A549 (lung carcinoma) and Fen (bladder carcinoma) by MTT assay.
Among studied cultures, hairy root culture of L. album showed the best cyototxic
activity on all cell lines. Callus culture of L. persicum and L. glaucom had
also significant cytotoxic activity on all cell lines. Normal root culture of L.
mucronatum showed weak cytotoxic activity on Jurkat and significant activity on
other cell lines. Hairy root culture of L. nodiflorum had no cytotoxic activity
on Jurkat but significant cytotoxicity on other cell lines. Hairy root culture
of L. catharticum showed no cytotoxicity on Jurkat, weak cytotoxicity on K562
and significant cytotoxic activity on other cell line.
The cyototoxic activity of Linum cultures investigated here could be due
to lignan compounds. Therefore, Linum cultures appear as valuable
candidates for isolation of cytotoxic compounds in large scales.
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