Asmari, S., Zafar, R., Ahmad, S. (2010). Production of sarsasapogenin from tissue culture of Asparagus racemosus and its quantification by HPTLC. Iranian Journal of Pharmaceutical Research, Volume 3(Supplement 2), 66-67.
S Asmari; R Zafar; S Ahmad. "Production of sarsasapogenin from tissue culture of Asparagus racemosus and its quantification by HPTLC". Iranian Journal of Pharmaceutical Research, Volume 3, Supplement 2, 2010, 66-67.
Asmari, S., Zafar, R., Ahmad, S. (2010). 'Production of sarsasapogenin from tissue culture of Asparagus racemosus and its quantification by HPTLC', Iranian Journal of Pharmaceutical Research, Volume 3(Supplement 2), pp. 66-67.
Asmari, S., Zafar, R., Ahmad, S. Production of sarsasapogenin from tissue culture of Asparagus racemosus and its quantification by HPTLC. Iranian Journal of Pharmaceutical Research, 2010; Volume 3(Supplement 2): 66-67.
Production of sarsasapogenin from tissue culture of Asparagus racemosus and its quantification by HPTLC
To develop an alternative method for production of sarsasapogenin by plant tissue culture technique and its quantification in Asparagus racemosus and its in vitro cultures using HPTLC.
Murashige and Skoog’s (MS) basal medium supplemented with various growth regulators was used for development of shoot and root calli while, hormone free MS medium was used for development of Agrobacterium tumefaciens (MTCC-532) mediated transformed shoot culture.
The quantification of sarsasapogenin was done using CAMAG HPTLC system, solvent system: Chloroform: Acetone (80:20) and spraying reagent: Anisaldehyde sulfuric acid at 365 nm wavelength.
The calli from shoot and root and morphogenetic shoot and root culture as well as Agrobacterium tumefaciens mediated transformed shoot culture of Asparagus racemosus was developed on MS medium.
The sarsasapogenin was found present in natural plant as well as in in vitro cultures. It was estimated quantitatively by CAMAG HPTLC system which, showed that highest amount (0.133%) was found to be present in shoot tumor followed by root callus (0.127%) that are 2.59 and 2.5 times higher respectively than natural root.
It can be concluded that the in vitro cultures produced by plant tissue culture technique have great potential for the production of higher amount of sarsasapogenin, which can be used for industrial scale up processes.
Full Text
Iranian Journal of Pharmaceutical Research (2004): Supplement 2
Iranian Journal of Pharmaceutical Research (2004): Supplement 2:
66-67
Poster Presentations/Biological Effect of
Medicinal Plants
2nd International Congress on Traditional Medicine and Materia Medica October 4-7, 2004, Tehran, Iran
199
Production of
sarsasapogenin from tissue culture of Asparagus racemosus and its
quantification by HPTLC
Asmari S., Zafar R., Ahmad S.
Plant Tissue
Culture Laboratory, Department of Pharmacognosy and Phytochemistry,
Faculty of
Pharmacy, Jamia Hamdard, New Delhi, India
To
develop an alternative method for production of sarsasapogenin by plant tissue
culture technique and its quantification in Asparagus racemosus and its
in vitro cultures using HPTLC.
Murashige and Skoog’s (MS) basal medium supplemented with various growth
regulators was used for development of shoot and root calli while, hormone free
MS medium was used for development of Agrobacterium tumefaciens (MTCC-532)
mediated transformed shoot culture.
The quantification of sarsasapogenin was done using CAMAG HPTLC system, solvent
system: Chloroform: Acetone (80:20) and spraying reagent: Anisaldehyde sulfuric
acid at 365 nm wavelength.
The calli from shoot and root and morphogenetic shoot and root culture as well
as Agrobacterium tumefaciens mediated transformed shoot culture of Asparagus
racemosus was developed on MS medium.
The sarsasapogenin was found present in natural plant as well as in in vitro
cultures. It was estimated quantitatively by CAMAG HPTLC system which, showed
that highest amount (0.133%) was found to be present in shoot tumor followed by
root callus (0.127%) that are 2.59 and 2.5 times higher respectively than
natural root.
It
can be concluded that the in vitro cultures produced by plant tissue culture
technique have great potential for the production of higher amount of
sarsasapogenin, which can be used for industrial scale up processes.
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