Iranian Journal of Pharmaceutical Research (2004): Supplement 2:
2nd International Congress on Traditional Medicine and Materia Medica
Effect of N- fertilizer and plant density on growth, development, herb yield and active substance of feverfew (Tanacetum parthenium Cv.Zardband) medicinal plant
Hassani Malayeri S.1, Omidbaigi R.1, Sefidkon F.2
1Department of Horticulture, School of Agriculture, Tarbiatmodarres University, Tehran, I.R.IRAN 2Research Institute of Forests and Rangelands, Medicinal Plants Department
The objective of this study was to investigate the effect of two N-fertilizer rates and plant density on herb yield and active substance of feverfew (Tanacetum parthenium Cv.Zardband).
The experiment was performed in a factorial trial, RCB design. Three rates of N-fertilizer (0.75 and 150 kg/ha) and plant densities of 10 and 20 cm were applied as treatments, with three replications.
After performing soil analysis of experimental site seedlings were transferred from nursery to the main plots.
Plants were harvested 5 cm from ground, when they reached 60% full-bloom, then air dried in laboratory. Dried samples constituents were characterized and quantified by means of water distillation extraction and GC/MS.
The results of Duncan multiple range test showed that, there is a very significant (P<0.01) difference between different levels of N-fertilizer and plant density for all factors. Each of 150 kg per ha of N-fertilizer and 20 cm space between plants separately resulted in higher plant fresh weight, plant dry weight and volume of essential oil, but the mutual effect of these two treatments was not significant. Also by applying these treatments, we obtained higher stem length, more stems and flowers per plants comparing with other treatments.
According to the comparison of means for essential oil constituents, 150 kg per ha of N-fertilizer and 10 cm space between plants resulted in a higher amount of α-pinene, camphen, p-cymen, limonene, β-pinene and α-thujene.
From the above results, we can conclude that by increasing N-fertilizer rate, more nutrition is provided for assimilation and by increasing plant density, more competition will happen, which both result in higher fresh and dry weigh and consequently higher amount of essential oil. For this trial and similar conditions 150 kg per ha N-fertilizer and 10 cm space between is recommended.