Iranian Journal of Pharmaceutical Research (2004): Supplement 2:
2nd International Congress on Traditional Medicine and Materia Medica
The study of effects of aqueous extracts of Zataria multiflora (ZM) and Elaeagnus angostifolia (EA) on the volume of stomach of mouse fetus
Mokhberi M., Shams Lahijani M., Monsefi M., Kamalinejad M.
Biology Depertment, Faculty of Sciences Shaheed Beheshti University, Tehran, Iran
ZM is a plant used to remedy dyspepsias in pregnant women. EA is a plant used as a stomach and heart tonic, carminative and in remedy of skeletal and muscular ailments. In order to investigate the probable effects of this extracts on the fetus digestive system the current study was examind.
Twenty-eight pregnant balb/c mice (25-30 gr) were divided into control, sham and expremental groups (n=7). From 6th to 15th gestation days, food and water of mouses were removed for about 3 hours. The expremental groups were gavaged with 200 mg/kg/day and 640 mg/kg/day aqueous extracts of ZM and EA in 0.5 ml distilled water, respectivly. The sham group was gavaged with equal amount of distilled water. Pregnant mice were dissected in 16th day of pregnancy. Crown-Rump length (CRL) and weight of them were measured. Seven fetuses from different mothers were selected. Five µm transverse serial sections with 75 µm interval were prepared. The Cavalieri method was applied to estimate the volume of different parts of stomach. The significant differences (p<0.01) between different volume of stomach were determined by Mann-Whitney U test and differences between Crown-Rump lenght and weight were applied by T-Test.
Statistical analysis showed no significant difference on volume of stomach by ZM but EA increased the volume of different parts of fetues stomach include volume of wall, epithelial layer, muscularis and serosa layer and total volume of stomach but no effecte in the lumen of stomach. No significant difference was seen for weight and CRL in expremental group. The coefficient error of Cavalieri method for these samples was between 1-9%.
ZA is not a teratogenic agent and does not show side effect on fetus growth. The useful effect of this plant on digestive system may be on mucosal cell of stomach that secreted acid and enzyme or done by unknown mechanisms. EA is probably effective via change in function of embryonic growth factors, gene expressions or in unknown mechanisms that increase the volume of stomach of fetuses.