Gilani, A., Bukhari, I., Alam Khan, R., Arif-ullah Khan, , Farman-ullah Jan, , Ahmad, V. (2010). Presence of cholinomimetic and calcium channel blocking constituents in Carthamus oxycantha. Iranian Journal of Pharmaceutical Research, Volume 3(Supplement 2), 54-54.
AH Gilani; IA Bukhari; R Alam Khan; Arif-ullah Khan; Farman-ullah Jan; VU Ahmad. "Presence of cholinomimetic and calcium channel blocking constituents in Carthamus oxycantha". Iranian Journal of Pharmaceutical Research, Volume 3, Supplement 2, 2010, 54-54.
Gilani, A., Bukhari, I., Alam Khan, R., Arif-ullah Khan, , Farman-ullah Jan, , Ahmad, V. (2010). 'Presence of cholinomimetic and calcium channel blocking constituents in Carthamus oxycantha', Iranian Journal of Pharmaceutical Research, Volume 3(Supplement 2), pp. 54-54.
Gilani, A., Bukhari, I., Alam Khan, R., Arif-ullah Khan, , Farman-ullah Jan, , Ahmad, V. Presence of cholinomimetic and calcium channel blocking constituents in Carthamus oxycantha. Iranian Journal of Pharmaceutical Research, 2010; Volume 3(Supplement 2): 54-54.
Presence of cholinomimetic and calcium channel blocking constituents in Carthamus oxycantha
The crude extract of Carthamus oxycantha (Co.Cr) was studied in vitro for its possible spasmogenic and spasmolytic activities.
Segments of isolated rabbit jejunum 2-3 cm long were mounted in Tyrode’s solution and aerated with 95% oxygen in carbon dioxide. Isotonic responses were measured on Bioscience oscillograph. Isotonic responses were measured on Bioscience oscillograph.
Co.Cr caused dose-dependent (0.03-3.0 mg/mL) spasmogenic effect in spontaneously contracting rabbit jejunum, followed by relaxation at the next higher doses of 5.0-10.0 mg/mL. In the presence of atropine (0.03 µM), spasmogenic effect was blocked and the relaxant effect was observed at lower doses (0.03-5.0 mg/mL). Co.Cr (1.0-10.0 mg/mL) produced relaxation of high K+ (80 mM)-induced contraction, suggestive of calcium channel blockade. The CCB effect was confirmed when Co.Cr in the atropinized tissue produced a dose-dependent shift in the Ca++ dose-response curves to the right, similar to that of verapamil, a standard calcium channel blocker.
These data indicate that Carthamus oxycantha exhibits spasmogenic effect through cholinergic action and the spasmolytic effect through calcium channel blockade.
Full Text
Iranian Journal of Pharmaceutical Research (2004): Supplement 2
Iranian Journal of Pharmaceutical Research (2004): Supplement 2:
54-54
Poster Presentations/Ethnopharmacology
2nd International Congress on Traditional Medicine and Materia Medica October 4-7, 2004, Tehran, Iran
158
Presence of
cholinomimetic and calcium channel blocking constituents in Carthamus
oxycantha
Gilani A.H.1, Bukhari
I.A.2, Alam Khan R.2, Arif-ullah Khan1,
Farman-ullah Jan3, Ahmad V.U.3
1Department
of Biological and Biomedical Sciences, The Aga Khan University, Stadium Road,
74800, Karachi, Pakistan, 2Department of Pharmacology, Faculty of
Pharmacy, University of Karachi, 3HEJ Research Institute of
Chemistry, University of Karachi, Pakistan
The crude extract of Carthamus oxycantha (Co.Cr) was studied in vitro for
its possible spasmogenic and spasmolytic activities.
Segments of isolated rabbit jejunum 2-3 cm long were mounted in Tyrode’s
solution and aerated with 95% oxygen in carbon dioxide. Isotonic responses were
measured on Bioscience oscillograph. Isotonic responses were measured on
Bioscience oscillograph.
Co.Cr caused dose-dependent (0.03-3.0 mg/mL) spasmogenic effect in spontaneously
contracting rabbit jejunum, followed by relaxation at the next higher doses of
5.0-10.0 mg/mL. In the presence of atropine (0.03 µM), spasmogenic effect was
blocked and the relaxant effect was observed at lower doses (0.03-5.0 mg/mL).
Co.Cr (1.0-10.0 mg/mL) produced relaxation of high K+ (80 mM)-induced
contraction, suggestive of calcium channel blockade. The CCB effect was
confirmed when Co.Cr in the atropinized tissue produced a dose-dependent shift
in the Ca++ dose-response curves to the right, similar to that of verapamil, a
standard calcium channel blocker.
These data indicate that Carthamus oxycantha exhibits spasmogenic effect
through cholinergic action and the spasmolytic effect through calcium channel
blockade.
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