Sleep promoting effects of BR-16A: interaction with GABAergic modulators



The aim of the present study was to investigate the sleep promoting effect of BR-16A, a polyherbal formulation along with GABAergic drugs. Pentobarbitone-induced hypnosis test was used as an animal model to explore its role in sleep. Pentobarbitone produces quick the sleep latency (onset) and prolongation of total sleep time (duration). Seep latency and total sleep time were used as parameters for the evaluation. BR-16A potentiated the effect of triazolam (0.1 mg/kg, ip) and alprazolam (0.25 mg/kg, ip). Melatonin (5.0 mg/kg, i.p.) and zolpidem (0.5 mg/kg, i.p.) did not produce any significant effect on sleep parameters (P<0.05). However alprazolam (0.25 mg/kg, i.p.) potentiated the effect of BR-16A (100 mg/kg, p.o.) only at higher doses. Sleep promoting effect of BR-16A, a polyherbal formulation in combination with GABAergic drugs (triazolam and alprazolam,) suggested that these drugs have common mechanism in sleep promoting effect of pentobarbitone and could be used along with other GABAergic hypnotics for the treatment of insomnia. This may reduce the applied dose of the latter drug(s). BR-16A can also be used for the treatment of sleep and related disorders.