Evaluation of the Toxicity Effects of Silk Fibroin on Isolated Fibroblast and Huvec Cells

Document Type: Research article

Authors

1 Pharmaceutical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

2 Department of Pharmaceutics, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran

3 Department of Medical Nanotechnology, Faculty of Advanced Technology in Medicine, Iran University of Medical Sciences, Tehran, Iran.

4 Department of Occupational Health Engineering, Alborz University of Medical Sciences, Karaj, Iran.

5 Department of Pharmacology and Toxicology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Abstract

Emerging line research showed that silk nanoparticles (NPs) have toxicity on the fibroblast
and Huvec cells without any toxicity recognized mechanisms. Recently, it suggested peripheral
arterial disease confounds almost eight million Americans. Also, due to the main effect of
fibroblast in a production of extracellular matrix (ECM), adhesive molecules, glycoproteins
and various cytokines, it decided to define the toxicity mechanistic of silk NPs in fibroblast
and Huvec cells based on oxidative stress markers. Therefore, it investigated whether silk
NPs is able to induce any abnormality in the fibroblast and Huvec cells based on reliable and
documented oxidative stress methods. Our results indicated that silk NPs (0.5, 1 and 2 mg/mL)
induces cellular and mitochondrial dysfunction including an increase in ROS production, lipid
peroxidation, mitochondria membrane potential (MMP) collapse, and oxidation of thiol groups
which caused to cytochrome c release. Besides, lysosomal integrity damage and decreased in
ATP/ADP ratio proposed disruptive effect of silk NPs on the mitochondrial respiratory chain
and cell death signaling induction.

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