The controlled release of dexamethasone sodium phosphate from bioactive electrospun PCL/gelatin nanofiber scaffold

Document Type: Research article

Authors

1 Department of chemical and petroleum engineering, Sharif University of technology, , Tehran, Iran.

2 Department of chemical and petroleum engineering, Sharif University of technology, Tehran, Iran.

3 Hearing Disorders Research Center, Loghman Hakim Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

4 Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical, Sciences Tehran, Iran.

Abstract

In this study, a system of dexamethasone sodium phosphate (DEXP)-loaded chitosan nanoparticles embedded in poly-ε-caprolacton (PCL) and gelatin electrospun nanofiber scaffold was introduced with potential therapeutic application for treatment of the nervous system. Besides anti-inflammatory properties, DEXP act through its glucocorticoid receptors, which are involved in the inhibition of astrocyte proliferation and microglial activation. Bovine serum albumin (BSA) was used to improve the encapsulation efficiency of DEXP within chitosan nanoparticles and to overcome its initial burst release. BSA incorporation within the chitosan nanoparticles increased the encapsulation efficiency of DEXP from 30% to 77%. The comparison between DEXP release profile from PCL/gelatin scaffold with and without chitosan nanoparticles revealed that the system of DEXP-BSA-loaded chitosan nanoparticles embedded in electrospun PCL nanofiber scaffold provided a more controlled release pattern of the loaded drug. The scaffolds properties in terms of structure, hydrophilicity, cell compatibility, mechanical property and biodegradability were further investigated, which might show its potential application for the repair of spinal cord injury.

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