Biological Evaluation of the Isolated Compounds from Methanol fraction of Leutea avicennia Mozaff.

Document Type: Research article

Authors

1 Department of Pharmacognosy, Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.

2 Depatrtment of medicinal chemistry, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

3 Department of Botany, University of Isfahan, Isfahan, Iran.

4 Depatrtmentof Drug and Food Control, and Pharmaceutical Quality Assurance Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.

Abstract

Leutea avicenniae Mozaff. belongs to Apiaceae family is an endemic species distributed in the west of Iran. The aim of this study was to investigate the antioxidant activity and acetylcholinestrase (AchE) inhibition of the crude extract, fractions and isolated compounds from methanolic fraction of L. avicenniae. Five compounds were detected from methanol fraction, three phenolic compounds as p-coumric acid, caffeic acid, salicylic acid and two flavonoids as quercetin and astragalin. These structures were identified by spectroscopic techniques such as 1H-NMR, 13C-NMR and UV.
Antioxidant activity was evaluated by the free radical scavenging assay using 2,2-diphenyl-1-picryl- hydrazyl (DPPH) method. Ellman colorimetric method was used to determine acetylcholinestrase (AChE) inhibition. In the DPPH assay, Quercetin exerted the highest antioxidant activity (IC50=10.24±1.3 µg/ml). Caffeic acid inhibited AChE with IC50 of 12.06±2.01µg/ml which were comparable to Galanthamine as positive control (IC50=62.44±2.2µg/ml).
In conclusion methanol extract of L. avicenniae contains bioactive components with antioxidant and AchE inhibitory effects.

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