Sabernavaei, M., Kobarfard, F., Hadjiakhoondi, A., Aghaahmadi, M., Amin, M., Yassa, N. (2018). Biological Evaluation of the Isolated Compounds from Methanol fraction of Leutea avicennia Mozaff.. Iranian Journal of Pharmaceutical Research, 17(4), 1386-1391. doi: 10.22037/ijpr.2018.2299
Mahsa Sabernavaei; Farzad Kobarfard; Abbas Hadjiakhoondi Hadjiakhoondi; Majid Aghaahmadi; Mohsen Amin; Narguess Yassa. "Biological Evaluation of the Isolated Compounds from Methanol fraction of Leutea avicennia Mozaff.". Iranian Journal of Pharmaceutical Research, 17, 4, 2018, 1386-1391. doi: 10.22037/ijpr.2018.2299
Sabernavaei, M., Kobarfard, F., Hadjiakhoondi, A., Aghaahmadi, M., Amin, M., Yassa, N. (2018). 'Biological Evaluation of the Isolated Compounds from Methanol fraction of Leutea avicennia Mozaff.', Iranian Journal of Pharmaceutical Research, 17(4), pp. 1386-1391. doi: 10.22037/ijpr.2018.2299
Sabernavaei, M., Kobarfard, F., Hadjiakhoondi, A., Aghaahmadi, M., Amin, M., Yassa, N. Biological Evaluation of the Isolated Compounds from Methanol fraction of Leutea avicennia Mozaff.. Iranian Journal of Pharmaceutical Research, 2018; 17(4): 1386-1391. doi: 10.22037/ijpr.2018.2299
Biological Evaluation of the Isolated Compounds from Methanol fraction of Leutea avicennia Mozaff.
1Department of Pharmacognosy, Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
2Depatrtment of medicinal chemistry, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
3Department of Botany, University of Isfahan, Isfahan, Iran.
4Depatrtmentof Drug and Food Control, and Pharmaceutical Quality Assurance Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Abstract
Leutea avicenniae Mozaff. belongs to Apiaceae family is an endemic species distributed in the west of Iran. The aim of this study was to investigate the antioxidant activity and acetylcholinestrase (AchE) inhibition of the crude extract, fractions and isolated compounds from methanolic fraction of L. avicenniae. Five compounds were detected from methanol fraction, three phenolic compounds as p-coumric acid, caffeic acid, salicylic acid and two flavonoids as quercetin and astragalin. These structures were identified by spectroscopic techniques such as 1H-NMR, 13C-NMR and UV. Antioxidant activity was evaluated by the free radical scavenging assay using 2,2-diphenyl-1-picryl- hydrazyl (DPPH) method. Ellman colorimetric method was used to determine acetylcholinestrase (AChE) inhibition. In the DPPH assay, Quercetin exerted the highest antioxidant activity (IC50=10.24±1.3 µg/ml). Caffeic acid inhibited AChE with IC50 of 12.06±2.01µg/ml which were comparable to Galanthamine as positive control (IC50=62.44±2.2µg/ml). In conclusion methanol extract of L. avicenniae contains bioactive components with antioxidant and AchE inhibitory effects.
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