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Iranian Journal of Pharmaceutical Research
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Eisa, N., Said, H., ElSherbiny, N., Eissa, L., El-Shishtawy, M. (2018). Phenethyl isothiocyanate Triggers Apoptosis, Combats Oxidative Stress and Inhibits Growth of Ehrlich Ascites Carcinoma Mouse Model. Iranian Journal of Pharmaceutical Research, 17(4), 1328-1338. doi: 10.22037/ijpr.2018.2289
Nada Eisa; Heba s. Said; Nehal M. ElSherbiny; Laila A. Eissa; Mamdouh El-Shishtawy. "Phenethyl isothiocyanate Triggers Apoptosis, Combats Oxidative Stress and Inhibits Growth of Ehrlich Ascites Carcinoma Mouse Model". Iranian Journal of Pharmaceutical Research, 17, 4, 2018, 1328-1338. doi: 10.22037/ijpr.2018.2289
Eisa, N., Said, H., ElSherbiny, N., Eissa, L., El-Shishtawy, M. (2018). 'Phenethyl isothiocyanate Triggers Apoptosis, Combats Oxidative Stress and Inhibits Growth of Ehrlich Ascites Carcinoma Mouse Model', Iranian Journal of Pharmaceutical Research, 17(4), pp. 1328-1338. doi: 10.22037/ijpr.2018.2289
Eisa, N., Said, H., ElSherbiny, N., Eissa, L., El-Shishtawy, M. Phenethyl isothiocyanate Triggers Apoptosis, Combats Oxidative Stress and Inhibits Growth of Ehrlich Ascites Carcinoma Mouse Model. Iranian Journal of Pharmaceutical Research, 2018; 17(4): 1328-1338. doi: 10.22037/ijpr.2018.2289

Phenethyl isothiocyanate Triggers Apoptosis, Combats Oxidative Stress and Inhibits Growth of Ehrlich Ascites Carcinoma Mouse Model

Article 17, Volume 17, Issue 4, Autumn 2018, Page 1328-1338  XML PDF (925.07 K)
Document Type: Research article
DOI: 10.22037/ijpr.2018.2289
Authors
Nada Eisa email 1; Heba s. Said2; Nehal M. ElSherbiny3; Laila A. Eissa3; Mamdouh El-Shishtawy email 3
1Department of Biochemistry, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt.
2Department of Microbiology, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt.
3Department of Biochemistry, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt.
Abstract
The aim of this study is to investigate the antitumor activity and possible molecular mechanism of Phenethyl isothiocyanate (PEITC) against Ehrlich ascites carcinoma in-vivo and in-vitro.
In-vivo, ascetic fluid volume, body weight, serum malondialdehyde (MDA) level and total antioxidant capacity (TAC) were determined using Ehrlich ascites carcinoma (EAC) bearing mice. In-vitro, MTT assay was used. RT-PCR was used to investigate role of PEITC in apoptosis by analyzing the expression of Bax, caspase-9, and Bcl-2 genes. The effect of PEITC on caspase-9 enzyme activity was also tested.
PEITC and/or Doxorubicin (Dox) treatment significantly suppressed EAC growth as compared to EAC/oil control mice. PEITC treatment showed a dose-dependent inhibition of EAC cells as indicated by MTT assay. We found that significant increase in MDA level and decrease in TAC caused by Dox treatment were significantly reduced by combination with PEITC treatment. Bax, caspase-9 genes’ expression and caspase-9 enzymatic activity were significantly increased, while Bcl-2 gene expression was significantly decreased in PEITC treated mice.
PEITC may act as a promising anticancer agent either alone or more effectively in combination with Dox through apoptotic cell death induction.
Keywords
Apoptosis; Bax; Bcl-2; Caspase-9; Ehrlich ascites carcinoma; Phenethyl isothiocyanate
Main Subjects
Pharmacy
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