Co -delivery of Sulforaphane and Curcumin with PEGylated Iron Oxide-Gold Core Shell Nanoparticles for delivery to breast cancer cell line

Document Type: Research article

Authors

1 Gene Therapy Research Center, Zanjan University of Medical Sciences

2 Biotechnology Research Center, Zanjan University of Medical Sciences

3 Department of Agricultural Manegement,Islamic Azad University, Abhar Branch,Zanjan,Iran.

4 School of Pharmacy, Zanjan University of Medical Sciences

Abstract

Co-delivery approach has been recommended to reduce the amount of each drug and to achieve the synergistic effect for cancer treatment. CUR and SF have antitumor effects, but their application is limited because of their low water solubility and poor oral bioavailability. To improve the bioavailability and solubility of SF and CUR, we performed an innovative co-delivery of SF and CUR with magnetic nanoparticles to endorse SF and CUR maintenance as an effective and promising antitumor drugs. The structure of the synthesized nanocarrieris evaluated by X-ray diffraction, transmission electron microscopy, scanning electron microscopy, vibrating sample magnetometer, dynamic light scattering and Fourier transform infrared spectroscopy. The results revealed that the zeta potential of CUR and SF-loaded NPs were about -15.4 mV and the average sizes were 80.57 nm. They were monodispersed (polydispersity index =0.161±0.016) in water, with high drug-loading capacity and stability. CUR and SF were encapsulated into NPs with loading capacity of 19.32 ± 0.023% and 18.74 ± 0.015% and entrapment efficiency of 83.72 ± 0.14% and 81.20 ± 0.18% respectively. The in-vitro study of SF and CUR loaded PEGylated Fe3O4@Au NPs on human breast adenocarcinoma cell line (MCF-7) confirmed that cytotoxicity of SF and CUR can enhance when they are loaded on PEGylated Fe3O4@Au NPs in comparison to free SF and CUR. The results of flow cytometry and real-time PCR shown that this combination can increase therapeutic effects of SF and CUR by apoptosis and necrosis induction as well as inhibiting of migration in MCF-7 cell line.

Keywords

Main Subjects