Document Type: Research article
Department of Pharmacognosy, Faculty of Pharmacy, Istanbul University, 34116 Istanbul, Turkey
Department of Biochemistry, Faculty of Pharmacy, Istanbul University, 34116 Istanbul, Turkey
Department of Pharmacognosy, Faculty of Pharmacy, Istanbul University, 34116 Istanbul, Turkey.
In this study, the antioxidant, antiacetylcholinesterase, anti-inflammatory, and DNA protecting activities of the aerial parts of Glaucium grandiflorum var. grandiflorum were evaluated. It was found that the lyophilized methanolic extract exhibited an inhibitory capacity against FeCl3/ascorbic acid induced posphatidylcholine liposome oxidation (EC50 = 2.62 0.08 mg/mL), quenched HOCl (5.87 0.07 mg/mL ), stable DPPH (EC50 = 2.03 0.17 mg/mL) and ABTS cation radicals (EC50 = 4.80 0.14 mg/mL) and acted as reductant as expressed by the FRAP value (2.453 0.07 mM/L Fe2+). The acethylcholinesterase inhibition capacity of the lyophilized methanolic extract at 320 μg/mL (80.75 1.59%) was found to be strong and almost equal to the inhibition capacity of the positive control, galantamine (82.23 2.21%) at 25 μg/mL. The significant AChE inhibitory activity suggests that the extract may be beneficial in the treatment of Alzheimer’s disease. The extract also showed inhibitory activity against plasmid DNA damage (94 %), as well as COX-2 (69.05 %), which is a target for many anti-inflammatory and cancer-preventive drugs. These results indicate that G. grandiflorum var. grandiflorum methanolic extract is an excellent source of compounds with antioxidant, anti-cholinesterase and anti-inflammatory properties that prevent DNA damage.