Document Type: Research article
Key Laboratory for Enzyme and Protein Technology, Hanoi University of Science, Vietnam.
Key Laboratory for Enzyme and Protein Technology, Hanoi University of Science, Vietnam
Faculty of Biology, Hanoi University of Science, Vietnam.
National centre for Technological progress, Vietnam.
Institute of scientifc research in military logistics/military academy of logistic, Vietnam.
Wedelolactone is known to have biological activities such as anti-inflammation hepatitis, anti-hepatotoxic activity, and trypsin inhibitory effect. However, up to date, there has not been studied deeply in the role of wedelolactone for zymosan-induced signaling pathways in the process of regulating the excessive inflammatory responses in host. Here, we demonstrated that wedelolactone plays an essential role for regulation of zymosan-induced inflammatory responses in murine bone marrow-derived macrophages (BMDMs). The zymosan-mediated secretion of tumor necrosis factor-α (TNF)-α), interleukin (IL)-6), and IL12p40 but not IL-10 in BMDMs was significantly inhibited by pre-treatment with wedelolactone (30 µg/ml, P < 0.001). Furthermore, zymosan-induced supreoxide generation, NADPH oxidase (P < 0.001), phosphorylation of p47phox in BMDMs were significantly reduced by pre-treatment of wedelolactone (30 µg/ml). Collectively, these data indicated that wedelolactone reduced zymosan-induced inflammatory responses. Moreover, in vivo wedelolactone (30 mg/kg) significantly rescued from zymosan-induced shock through inhibition of systemic inflammatory cytokine levels.