11. Animal Developmental Biology, Research center for Animal Development Applied Biology& Dept. Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran
2kharazmi university,Tehran, Iran
3Research center for animal Development applied Biolody,Mashhad Branch,Islamic azad University
4Research center for animal Development Applied Biology,Mashhad Branch,Islamic Azad University,Mashhad Iran
Abstract
Aims: The biosynthesis of nanoparticles is widely considered today. The aim of this investigation was the biosynthesis and coating of Ag.NPs with Zataria multiflora (Zm-Ag.NPs) leaf extract and assess its apoptosis promoting effects. Methods: The Zm-Ag.NPs was characterized by UV-visible and FTIR spectroscopy, TEM, EDS, DLS and measurement of zeta-potential. Apoptosis induction effects of Zm-Ag.NPs were assessed using acridine orange – propidium iodide (AO/PI) and DAPI staining, caspase3/9 activation assay and annexinV/PI assay. We also assess changing on P53, matrix metalloproteinases 2 (MMPs) and vascular endothelial growth factor A (VEGF-A) genes expression with semi-quantitative RT-PCR. Results: The UV-visible spectroscopy results showed that the surface plasmon resonance band (SRP) for Zm-Ag.NPs was around 440 nm also FTIR spectroscopy indicated plant material embedded around of Zm-Ag.NPs. The TEM images of the samples revealed that the Ag.NPs varied in morphology also presence of the silver element was monitories with EDS. the mean size of Zm-Ag.NPs were 30 nm. The Zm-Ag.NPs decrease cell viability in a dose and time dependent manner (IC50 = 15 μg/ml). AO/PI and DAPI staining indicated chromatin fragmentation also annexinV externalization assay using flow cytometer confirm promoting programmed cell death in treated cells. Apoptosis induced through caspase 3/ 9 activation pathway. This promoting apoptosis effects didn’t relate with P53 gene up regulation. Finally, it was found that Zm-Ag.NPs inhibited cancer cell metastasis through a decrease in MMP and VEGFA expression. Conclusion: Zm-Ag.NPs acts as carrier for plant material compound that can apply as anticancer agents.
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