Document Type: Research article
Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
Student Research Committee, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
<I>Nepeta</I> (Lamiaceae) is an important genus with beneficial medicinal properties. N. sintenisii
Bornm. has been used in folk medicine of Iran to cure various diseases. We investigated the
anti-melanogenesis effects of n-hexane, MeOH, CH2Cl2, n-BuOH, EtOAc, and H2O extracts
isolated from the plant in B16 melanoma cells. Various assays including cytotoxicity, mushroom
tyrosinase inhibition, inhibition of cellular tyrosinase, melanin content, the amount of reactive
oxygen species and western blotting were done to assess the plant activities on melanogenesis
inhibition. All extracts of N. sintenisii could significantly reduce both tyrosinase activity
and the cellular melanin content. Reactive oxygen species were also significantly decreased
following the treatment of cell with n-BuOH and EtOAc extracts with no cytotoxicity. The plant
significantly decreased the amount of microphthalmia-associated transcription factor proteins.
Collectively, N. sintenisii inhibited melanin synthesis and tyrosinase activity in B16 melanoma
cells with no cytotoxic effects. Hence, it might merit further investigations for elucidation of