Defensive Properties of Ginsenoside Re against UV-B-Induced Oxidative Stress through Up-Regulating Glutathione and Superoxide Dismutase in HaCaT Keratinocytes

Document Type: Research article

Authors

1 Research and Development Center, Cosmocos Corporation, Incheon 21698, Korea.

2 Department of Biochemistry, College of Natural Sciences, Kangwon National University, Chuncheon 24341, Korea.

3 Department of Biological Sciences, College of Natural Sciences, Kangwon National University, Chuncheon 24341, Korea.

4 College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 08826, Korea.

Abstract

Ginseng is now used worldwide as a traditional Oriental medicine. Ginsenosides, also
known as ginseng saponins, are responsible for most pharmacological efficacies of ginseng.
This work aimed to assess the novel skin anti-photoaging potential of ginsenoside Re (Re), a
protopanaxatriol-type ginsenoside, by analyzing reactive oxygen species (ROS), pro-matrix
metalloproteinase-2 (proMMP-2) and -9 (proMMP-9), total glutathione (GSH), superoxide
dismutase (SOD), and cellular viability in UV-B-irradiated HaCaT keratinocytes. When HaCaT
cells were pretreated with Re prior to UV-B irradiation, Re significantly suppressed the UVB-
induced ROS elevation. It was also able to attenuate the UV-B-induced proMMP-2 and -9
elevations at both activity and protein levels. Re was capable of overcoming the UV-B-reduced
total GSH content and SOD activity in concentration-dependent ways. Under the experimental
conditions used, Re could interfere with cellular viabilities in neither non-irradiated nor UV-Birradiated
keratinocytes.

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