Document Type: Research article
Research and Development Center, Cosmocos Corporation, Incheon 21698, Korea.
Department of Biochemistry, College of Natural Sciences, Kangwon National University, Chuncheon 24341, Korea.
Department of Biological Sciences, College of Natural Sciences, Kangwon National University, Chuncheon 24341, Korea.
College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 08826, Korea.
Ginseng is now used worldwide as a traditional Oriental medicine. Ginsenosides, also
known as ginseng saponins, are responsible for most pharmacological efficacies of ginseng.
This work aimed to assess the novel skin anti-photoaging potential of ginsenoside Re (Re), a
protopanaxatriol-type ginsenoside, by analyzing reactive oxygen species (ROS), pro-matrix
metalloproteinase-2 (proMMP-2) and -9 (proMMP-9), total glutathione (GSH), superoxide
dismutase (SOD), and cellular viability in UV-B-irradiated HaCaT keratinocytes. When HaCaT
cells were pretreated with Re prior to UV-B irradiation, Re significantly suppressed the UVB-
induced ROS elevation. It was also able to attenuate the UV-B-induced proMMP-2 and -9
elevations at both activity and protein levels. Re was capable of overcoming the UV-B-reduced
total GSH content and SOD activity in concentration-dependent ways. Under the experimental
conditions used, Re could interfere with cellular viabilities in neither non-irradiated nor UV-Birradiated