Crude Methanol Extract of Echinophora Platyloba Induces Apoptosis and Cell Cycle Arrest at S-Phase in Human Breast Cancer Cells

Document Type: Research article

Authors

1 Department of Cellular and Molecular Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran.

2 Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.

Abstract

The aim of the present study was to determine cytotoxic activity of crude methanolic
extract of Echinophora platyloba on breast cancer MDA-MB-231 cell line. The free radical
scavenging effects of methanolic extract of E. platyloba were tested using DPPH method.
Crude methanolic extract exhibited potential antioxidant activity with an IC50 value of 234.28 ±
21.63 μg/mL when compared to the standard BHT with an IC50 value of the 19.5 ± 0.8 μg/mL.
In addition, the in-vitro cytotoxic activity of this extract was studied against MDA-MB-231
and MCF-10a cells by MTT assay for 12, 24 and 36 h. Our data showed 534.6 ± 7.2 μg/mL of
extract following 24 h of incubation was the most cytotoxic dose against MDA-MB-231 cells
in comparison with other doses. This extract could induce apoptosis and promote cell-cycle
arrest at S-phase in MDA-MB-231 cells after 24 h of incubation, as compared to the control
group (p < 0.001) and could significantly up-regulate the expression of bax and p27 genes at
the level of 2.8 and 2.2 folds, respectively. While, a significant amount of down-regulation was
observed for bcl-2 gene expression, which was observed to be 0.4 fold. The present results
prove the anticancer capacity of crude methanolic extract of E. platyloba to inhibit limit cell
proliferation, and inducing cell cycle arrest and apoptosis.

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