Evaluation of Silibinin effects on the Viability of HepG2 (Human hepatocellular liver carcinoma) and HUVEC (Human Umbilical Vein Endothelial) cell lines

Document Type: Research article

Authors

1 Department of Toxicology and Pharmacology, school of Pharmacy, Shahid Beheshti University of Medical Science, Tehran, Iran

2 Cancer research Center, Shohadae Tajrish Hospital, Department of Radiation Oncology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

3 1 Department of Toxicology and Pharmacology, school of Pharmacy, Shahid Beheshti University of Medical Science, Tehran, Iran. 2 SBMU Pharmaceutical Sciences Research Center, Tehran, Iran

4 Cancer research center,Shohadae Tajrish Hospital, Department of Radiation Oncology, Shahid Beheshti University of Medical Sciences

Abstract

Human hepatocellular carcinoma is one of the most common recurrent malignancies, for as much as, there is no effective therapy. Silibinin, a widely used drug and supplement for various liver disorders, demonstrated anticancer effects against human hepatocellular carcinoma, human prostate adenocarcinoma cells, human breast carcinoma cells, human ectocervical carcinoma cells, and human colon cancer cells. Considering the antihepatotoxic activity of silibinin and its strong preventive and anticancer efficacy against various epithelial cancers, we investigated the efficacy of silibinin against human HCC and HUVEC cell lines. Silibinin effects on the growth and mode of cell death of these two cell lines are presented in this paper. HepG2 and HUVEC cells were incubated with different doses of silibinin (12.5, 25, 50, 100, 150 and 200 μg/ml) at 24, 48 and72 hours. Cytotoxicity was assessed using MTT and Trypan blue assays. Mode of cell death induced by silibinin was investigated using LDH assay and acridine orange/PI double dye staining. The results showed that silibinin has dose-dependent inhibitory effect on the viability of HepG2 and HUVEC cells. However, Silibinin causes a more continuous dose-dependent cytotoxicity in HepG2 cells compare to the HUVEC cells in which some degrees of resistance is apparent at the beginning. The mode of cell death looks also different in these two cell lines with HepG2 cells being more in favor of apoptosis while necrosis is more evident for the HUVEC cells.

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