Sharafi, Z., Barati, M., Khoshayand, M., Adrangi, S. (2017). Screening for Type II L-Asparaginases: Lessons from the Genus Halomonas. Iranian Journal of Pharmaceutical Research, 16(4), 1565-1573. doi: 10.22037/ijpr.2017.2145
Zeinab Sharafi; Mahmood Barati; Mohammadreza Khoshayand; Sina Adrangi. "Screening for Type II L-Asparaginases: Lessons from the Genus Halomonas". Iranian Journal of Pharmaceutical Research, 16, 4, 2017, 1565-1573. doi: 10.22037/ijpr.2017.2145
Sharafi, Z., Barati, M., Khoshayand, M., Adrangi, S. (2017). 'Screening for Type II L-Asparaginases: Lessons from the Genus Halomonas', Iranian Journal of Pharmaceutical Research, 16(4), pp. 1565-1573. doi: 10.22037/ijpr.2017.2145
Sharafi, Z., Barati, M., Khoshayand, M., Adrangi, S. Screening for Type II L-Asparaginases: Lessons from the Genus Halomonas. Iranian Journal of Pharmaceutical Research, 2017; 16(4): 1565-1573. doi: 10.22037/ijpr.2017.2145
Screening for Type II L-Asparaginases: Lessons from the Genus Halomonas
1Department of Pharmaceutical Biotechnology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2Department of Drug and Food Control, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
Abstract
Among the two types of bacterial L-asparaginases, only type II enzymes have been used in the treatment of acute lymphoblastic leukemia owing to their higher affinity for L-asparagine. However, current screening media used for the isolation of L-asparaginase-producing microorganisms do not discriminate between the two types of L-asparaginase. During an optimization study conducted to increase L-asparaginase production by environmental Halomonas isolates, it was noticed that the pattern of L-asparaginase production in response to variations in glucose concentration varied between different isolates suggesting that they differ in their ability to produce type II L-asparaginases, an observation that was confirmed by further experiments. Bioinformatics analysis of available Halomonas whole genome sequences revealed that indeed some species of this genus possess both L-asparaginase types while others possess only type I enzymes. By comparing the growth pattern of these isolates on different media, we propose that by omitting glucose, reducing the concentration of L-asparagine and providing an alternative nitrogen source in L-asparaginase screening media it may be possible to differentiate between type I and type II activities.
Top