Document Type : Research article
Department of Pharmacology, School of Medicine, Shahed University, Tehran, Iran.
Neuroscience Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, Iran.
School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
School of Medicine, Shahed University, Tehran, Iran.
Experimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
The present study was conducted to investigate the possible anxiolytic and sedative of
an acute administration and 4-day repeated dosing of an aqueous extract of flowers of Alcea
aucheri (Boiss.) Alef. (EFA) in rats subjected to the elevated plus-maze (EPM), open-field, and
horizontal wire tests. All drugs were administered intraperitoneally. Phytochemical screening
confirmed the presence of phenolic compounds, flavonoids, and polysaccharides in the extract.
Repeated dosing of EFA (at dose of 35 mg/kg) significantly increased percentage of time
spent on open arms and of open arms entries, and also decreased percentage of time spent on
closed arms and of closed arms entries; compared with saline control, 24 h after treatment.
In addition, repeated dosing of EFA (at dose of 175 mg/kg) significantly increased open arm
activity 48 h after treatment, versus saline group. This effect was also observed following acute
administration of EFA at 175 mg/kg. In open field, acute administration of EFA at doses of 17.5,
35, 70, 175, 350, and 700 mg/kg induced a statistically significant and dose-dependent decrease
in locomotor activity, compared with saline control. ED50 value for EFA-induced decrease in
locomotor activity was 194 mg/kg. Furthermore, unlike diazepam; EFA didn´t decrease the
percent of the rats grasping the wire.
These data suggest that Alcea aucheri extract may have anxiolytic and sedative properties
and some of the components in the extract such as phenolic compounds may have contributed
to the observed effects