Design and Fabrication of a Novel Transplant Combined with Human Bone Marrow Mesenchymal Stem Cells and Platelet-rich Fibrin: New Horizons for Periodontal Tissue Regeneration after Dental Trauma

Document Type: Research article

Authors

1 Department of Pediatric Dentistry, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran

2 Postgraduate Student, Department of Pediatric Dentistry, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran

3 Transplant Research Center, Shiraz university of medical sciences, Shiraz, Iran; Shiraz Stem cell institute for cell therapy and regenerative medicine, Shiraz University of Medical Sciences, Shiraz, Iran

Abstract

Avulsed teeth that are replanted dried are more prone to loss. Recent tissue engineering studies focus on
fabrication of various cell delivery systems to improve the overall prognosis of such teeth. To evaluate this new
cell transplant method, we initially aimed at designing of PRF scaffold and determining BMMSCs viability
and function on the fabricated scaffold. To test this concept in-vitro, human BMMSCs were isolated and
characterized by cell surface marker, and their potential on osteogenic/adipogenic differentiation. The biological
effects of PRF scaffold on human BMMSCs were then investigated by cell proliferation assay. The data were
quantified for statistical analysis. It was found that PRF significantly induced BMMSCs proliferation throughout
the incubation period due to its growth factor secretion. Results of MTT assay showed an increasing trend during
the testing period from day 1 to day 7. The result of scanning electron microscopy showed that BMMSCs could
tightly adhere to fibrin scaffold just shortly after seeding. These data suggest that the BMMSCs/PRF construct
has the potential to improve the clinical prognosis of replanted avulsed teeth in future. Additional studies are
needed to be performed before its clinical use.

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