Impact of Aspirin Eugenol Ester on Cyclooxygenase-1,Cyclooxygenase-2,C-reactive protein,Prothrombin and Arachidonate 5-lipoxygenase in Healthy Rats

Document Type: Research article

Authors

1 Key Lab of New Animal Drug Project of Gansu Province, Key Lab of Veterinary Pharmaceutical Development, Ministry of Agriculture, Lanzhou Institute of Husbandry and Pharmaceutical Science of CAAS, Lanzhou730050, P.R. China.

2 School of Pharmacy Lanzhou University, Lanzhou 730020, P.R. China.

Abstract

Aspirin eugenol ester (AEE) is a promising drug candidate, which is used for the treatment of inflammation, pain and fever and the prevention of cardiovascular diseases. This study focuses on the effect of AEE on five proteins which are related to inflammation and thrombosis, including cyclooxygenase-1 (COX-1), cyclooxygenase-2 (COX-2), C-reactive protein (CRP), prothrombin (FII) and arachidonate 5-lipoxygenase (ALOX5). Meanwhile, the study was administrated to compare the drug effect between AEE and its precursor from the view of chemical-protein interactions. Healthy rats were given AEE, aspirin, eugenol and integration of aspirin and eugenol. Carboxyl methyl cellulose sodium (CMC-Na) was used as control. After drugs were administered intragastrically for seven days, the blood samples were collected to measure the proteins concentration by enzyme linked immuno-sorbent assay (ELISA). The results showed that the concentrations of key endogenic bioactive enzymes are significantly reduced in AEE groups when compared with CMC-Na and aspirin groups (P<0.01). Drug effects of AEE on five proteins were stronger than aspirin and eugenol. From the view of chemical-protein interactions, AEE had positive effects on anti-inflammation and anti-thrombosis and showed stronger effects than aspirin and eugenol.

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