Toxicity Assessment of Asteraceae Centaurea Repens L Extract in Mice

Document Type : Research article


1 Pharmaceutical Sciences Research Center, Islamic Azad University (IAUPS), Pharmaceutical Sciences Branch, Tehran, Iran.

2 Department of Pharmacognosy, School of Pharmacy, Shahid Beheshti University of Medical Sciences and Health Services, Tehran, Iran.

3 Department of Toxicology & Pharmacology, Islamic Azad University, Pharmaceutical Sciences Branch (IAUPS), Tehran, Iran.


The species Asteraceae Centaurea repens (Asteraceae), known as Acroptilon repens, and
Talkhe in persian is used in folk medicine as an emetic, anti-epileptic, and anti-malaria herb
in many parts of the world but its toxic effects have not determined yet. This study aimed to
evaluate the acute and subchronic toxicity of this extract to find its possible adverse health
effects through clinical, hematological, biochemical, and histopathological endpoints in both
gender of mice. Aerial parts of the plant were air-dried and the terpene extract of aerial parts
of plant was provided by percolation using methanol, petroleum ether, and diethyl ether. All
clinical, biochemical and histopathological changes were assessed in appropriate endpoints and
compared with control group. Although no mortality was seen in acute study by administrating
doses up to 2000 mg/kg, repeated dose study on 1000 mg/kg doses in 28 days in both genders
showed liver necrosis and rise of liver enzymes (p-value < 0.05). Histopathological studies
didn’t show any other organ toxicity in dosed up to 1000 mg/kg. At the same time this study
showed for the first the antihyperlipidemic properties of the aerial extract of Acroptilin in mice
model. The pharmacological and histopathological results of the present study proved that the
total parts of Acroptilon repens could be studied for supporting the traditional assertion in folk
medicine to heal hyperlipidemia, diabetes, and cancer in lower doses although we performed
the present study and concluded liver toxicity by subchronic use of Acropitolon repens extract.


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