Anticancer Activity of Curcumin-Loaded PLGA Nanoparticles on PC3 Prostate Cancer Cells

Document Type: Research article

Authors

1 Cell and Molecular Research Center, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

2 Targeted Drug Delivery Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.

3 Department of Immunology and Cancer, Petroleum Pollutants, Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

4 Cell and Molecular Research Center, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

Abstract

Curcumin (Cur) has been found to be very efficacious against many different types of cancer
cells. However, the major disadvantage associated with the use of Cur is its low systemic
bioavailability. Our present work investigated the toxic effect of encapsulation of Cur in PLGA
(poly lactic-coglycolic acid) nanospheres (NCur) on PC3 human cancer prostate cell. In the
present study, we have investigated the effects of NCur on growth, autophagia, and apoptosis
in PC3 cells, respectively, by MTT assay, fluorescence microscopy, and Flow cytometry. MTT
assays revealed that the NCur at the concentration of 25 μg/mL for 48 h were able to exert a more
pronounced effect on the PC3 cells as compared to free Cur. Apoptotic index was significantly
increased in NCur-treated cells compared to free Cur. The percentage of autophagic cells (LC3-
II positive cells) was also significantly increased in NCur treatment in comparison to free Cur.
These data indicate that the NCur has considerable cytotoxic activity more than Cur on PC3
cell lines, which is mediated by induction of both apoptotic and autophagic processes. Thus,
NCur has high potential as an adjuvant therapy for clinical application in prostate cancer.

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