Evaluation of a 99mTc-tricine Vascular Disrupting Agent as an In-vivo Imaging in 4T1 Mouse Breast Tumor Model

Document Type: Research article

Authors

Radiation Application Research School, Nuclear Science and Technology Research Institute (NSTRI), Tehran, Iran.

Abstract

Colchicine as a vascular disrupting agent creates microtubule destabilization which
induces vessel blockage and consequently cell death. Accordingly, colchicines and its
analogues radiolabeled with 99mTc may have potential for visualization of tumor. In this work,
deacetylcolchicine a colchicine analogue was labeled with 99mTc via tricine as a coligand
and characterized for its tumor targeting properties. The in-vitro radiochemical stability and
the biodistribution were studied in 4T1 breast tumor model bearing mice. Labeling yield of
more than 90% was obtained corresponding to a specific activity of 46 MBq/μmol. In-vivo
biodistribution studies demonstrated that radiocomplex had high tumor to muscle and tumor to
blood ratios at early time points. Planer gamma imaging of tumor bearing mice showed that this
radioconjugate was able to clearly visualize tumors. According to high tumor uptake, presented
radiocomplex may have a potential for targeted imaging studies.

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