• Home
  • Browse
    • Current Issue
    • By Issue
    • By Author
    • By Subject
    • Author Index
    • Keyword Index
  • Journal Info
    • About Journal
    • Aims and Scope
    • Editorial Board
    • Editorial Staff
    • Publication Ethics
    • Indexing and Abstracting
    • Related Links
    • FAQ
    • Peer Review Process
    • News
  • Guide for Authors
  • Submit Manuscript
  • Reviewers
  • Contact Us
 
  • Login
  • Register
Home Articles List Article Information
  • Save Records
  • |
  • Printable Version
  • |
  • Recommend
  • |
  • How to cite Export to
    RIS EndNote BibTeX APA MLA Harvard Vancouver
  • |
  • Share Share
    CiteULike Mendeley Facebook Google LinkedIn Twitter Telegram
Iranian Journal of Pharmaceutical Research
Articles in Press
Current Issue
Journal Archive
Volume Volume 18 (2019)
Volume Volume 17 (2018)
Volume Volume 16 (2017)
Issue Issue 4
Issue Issue 3
Issue Issue 2
Issue Special Issue
Issue Issue 1
Volume Volume 15 (2016)
Volume Volume 14 (2015)
Volume Volume 13 (2014)
Volume Volume 12 (2013)
Volume Volume 11 (2012)
Volume Volume 10 (2011)
Volume Volume 9 (2010)
Volume Volume 8 (2009)
Volume Volume 7 (2008)
Volume Volume 6 (2007)
Volume Volume 5 (2006)
Volume Volume 4 (2005)
Volume Volume 3 (2004)
Volume Volume 2 (2003)
Volume Volume 1 (2002)
Khosravi, M., Rahimi, R., pourahmad, J., Zarei, M., Rabbani, M. (2017). Comparison of kinetic study and protective effects of biological dipeptide and two porphyrin derivatives against metal cytotoxicity in human lymphocytes. Iranian Journal of Pharmaceutical Research, 16(3), 1059-1070.
Maryam Khosravi; Rahmatollah Rahimi; Jalal pourahmad; Mohammad Hadi Zarei; Mahboubeh Rabbani. "Comparison of kinetic study and protective effects of biological dipeptide and two porphyrin derivatives against metal cytotoxicity in human lymphocytes". Iranian Journal of Pharmaceutical Research, 16, 3, 2017, 1059-1070.
Khosravi, M., Rahimi, R., pourahmad, J., Zarei, M., Rabbani, M. (2017). 'Comparison of kinetic study and protective effects of biological dipeptide and two porphyrin derivatives against metal cytotoxicity in human lymphocytes', Iranian Journal of Pharmaceutical Research, 16(3), pp. 1059-1070.
Khosravi, M., Rahimi, R., pourahmad, J., Zarei, M., Rabbani, M. Comparison of kinetic study and protective effects of biological dipeptide and two porphyrin derivatives against metal cytotoxicity in human lymphocytes. Iranian Journal of Pharmaceutical Research, 2017; 16(3): 1059-1070.

Comparison of kinetic study and protective effects of biological dipeptide and two porphyrin derivatives against metal cytotoxicity in human lymphocytes

Article 20, Volume 16, Issue 3, Summer 2017, Page 1059-1070  XML PDF (1147 K)
Document Type: Research article
Authors
Maryam Khosravi1; Rahmatollah Rahimi* 1; Jalal pourahmad* 2; Mohammad Hadi Zarei2; Mahboubeh Rabbani1
1Bionorganic Chemistry Research Laboratory, Chemistry Department, Iran University of Science and Technology, Narmak, Tehran, Iran
2School of Pharmacy, Pharmaceutical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Abstract
In this research, dipeptide (his-β-alanine) and porphyrin derivatives were choosen for comparing chelating ability of toxic metals such as Al3+, Cu2+, Hg2+ and Pb2+ in vitro. The reason for choosing these two compounds is that both of them are naturally present in biological systems and comparison of chelating ability of these two compounds has not yet been done. Synthesis and comparison of kinetic study of dipeptide (his-β-alanine), meso-tetrakis(4-trimethylanilinium) porphyrin (TAPP) and Tetrakis(4-sulfonatophenyl)porphyrin (TPPS4) were carried out by our team. In addition, cytotoxicity assays of metals and chelators were also performed using methylthiazoletetrazolium (MTT) test. Furthermore we investigated the protective effect of chelators against cytotoxicity, induced by differenrt toxic metals such as Al3+, Cu2+, Hg2+ and Pb2+ on human lymphocytes. EC50 values on humanlymphocytes obtained after 12 h incubation for Al3+, Cu2+ and Hg2+ were 30, 51, 3 µM respectively and for Pb2+ no cytotoxicity was observed on human lymphocyte up to 1000 µM concentration. EC50 obtained for chelators dipeptide, TPPS4 and TAPP were 948, 472 and 175 µM respectively. Pretreatment of human lymphocyte with subtoxic concentations of chelators reduced toxicity of the metals against human blood lymphocytes.
Keywords
Dipeptide; Porphyrin; Chelator; Toxic metals; Cytotoxicity; EC50; lymphocytes
Main Subjects
toxicology and Pharmacology
Statistics
Article View: 2,066
PDF Download: 53,758
Home | Glossary | News | Aims and Scope | Sitemap
Top Top

Statistics

Journal Management System. Designed by sinaweb.