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Pourahmad Jaktaji, R., Zarghampoor, F. (2017). Expression of tolC and organic solvent tolerance of Escherichia coli ciprofloxacin resistant mutants. Iranian Journal of Pharmaceutical Research, 16(3), 1185-1189. doi: 10.22037/ijpr.2017.2077
Razieh Pourahmad Jaktaji; Farzaneh Zarghampoor. "Expression of tolC and organic solvent tolerance of Escherichia coli ciprofloxacin resistant mutants". Iranian Journal of Pharmaceutical Research, 16, 3, 2017, 1185-1189. doi: 10.22037/ijpr.2017.2077
Pourahmad Jaktaji, R., Zarghampoor, F. (2017). 'Expression of tolC and organic solvent tolerance of Escherichia coli ciprofloxacin resistant mutants', Iranian Journal of Pharmaceutical Research, 16(3), pp. 1185-1189. doi: 10.22037/ijpr.2017.2077
Pourahmad Jaktaji, R., Zarghampoor, F. Expression of tolC and organic solvent tolerance of Escherichia coli ciprofloxacin resistant mutants. Iranian Journal of Pharmaceutical Research, 2017; 16(3): 1185-1189. doi: 10.22037/ijpr.2017.2077

Expression of tolC and organic solvent tolerance of Escherichia coli ciprofloxacin resistant mutants

Article 33, Volume 16, Issue 3, Summer 2017, Page 1185-1189  XML PDF (249.75 K)
Document Type: Research article
DOI: 10.22037/ijpr.2017.2077
Authors
Razieh Pourahmad Jaktaji email 1; Farzaneh Zarghampoor2
1Assistant professor in Genetics at University of Shahrekord
2Ph D student at Shaheed Chamran Uni.
Abstract
AcrAB-TolC is a major efflux pump in Escherichia coli. It was reported that tolC is overexpressed and involves in improving the organic solvent tolerance level in Escherichia coli marR mutants that are resistant to several antibiotics, such as ciprofloxacin. Low and intermediate levels resistance did not improve organic solvent tolerance. Thus, in this descriptive-experimental study it was decided to measure tolC expression and organic solvent tolerance in high level ciprofloxacin resistant mutants. tolC expression was measured by real time PCR and organic solvent tolerance assay was conducted by counting bacterial colonies on LBGMg agar. Results showed that tolC expression was increased significantly (P<0.05) and organic solvent tolerance was slightly improved in high resistant mutants. It was concluded that high organic solvent tolerance may need higher expression of tolC.
Keywords
AcrAB-TolC efflux pump; Ciprofloxacin; E. coli; Organic solvent tolerance; tolC; marR
Main Subjects
Drug quality control
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