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Sotoudegan, F., Amini, M., Faizi, M., Aboofazeli, R. (2017). Development of an RP-HPLC-UV method for simultaneous detection of nimodipine and its metabolite in cerebrospinal fluid of rat. Iranian Journal of Pharmaceutical Research, 16(2), 471-477. doi: 10.22037/ijpr.2017.2054
Farzaneh Sotoudegan; Mohsen Amini; Mehrdad Faizi; Reza Aboofazeli. "Development of an RP-HPLC-UV method for simultaneous detection of nimodipine and its metabolite in cerebrospinal fluid of rat". Iranian Journal of Pharmaceutical Research, 16, 2, 2017, 471-477. doi: 10.22037/ijpr.2017.2054
Sotoudegan, F., Amini, M., Faizi, M., Aboofazeli, R. (2017). 'Development of an RP-HPLC-UV method for simultaneous detection of nimodipine and its metabolite in cerebrospinal fluid of rat', Iranian Journal of Pharmaceutical Research, 16(2), pp. 471-477. doi: 10.22037/ijpr.2017.2054
Sotoudegan, F., Amini, M., Faizi, M., Aboofazeli, R. Development of an RP-HPLC-UV method for simultaneous detection of nimodipine and its metabolite in cerebrospinal fluid of rat. Iranian Journal of Pharmaceutical Research, 2017; 16(2): 471-477. doi: 10.22037/ijpr.2017.2054

Development of an RP-HPLC-UV method for simultaneous detection of nimodipine and its metabolite in cerebrospinal fluid of rat

Article 8, Volume 16, Issue 2, Spring 2017, Page 471-477  XML PDF (384.5 K)
Document Type: Research article
DOI: 10.22037/ijpr.2017.2054
Authors
Farzaneh Sotoudegan1; Mohsen Amini2; Mehrdad Faizi1; Reza Aboofazeli email 3
1School of Pharmacy, Shahid Beheshti University of Medical Sciences
2Faculty of Pharmacy, Tehran University of Medical Sciences
3Department of Pharmaceutics, School of Pharmacy, Shahid Beheshti Uni. Med. Sci.
Abstract
A rapid, simple and reproducible HPLC method was developed and validated for the analysis of nimodipine (NM) and/or its metabolite, oxidized nimodipine (OX–NM) in rat cerebrospinal fluid (CSF) and artificial CSF. The NM and OX–NM were eluted in less than 10 min with no interferences from the endogenous CSF peaks. Analysis was carried out on a Eurospher Performance (RP-C18, 250 × 4.6 mm) column and UV detection at 236 nm. The mobile phase consisted of acetonitrile and water (70:30 v/v, respectively) with a flow rate of 1 mL/min. Limit of detection was 0.1 μg/mL for OX-NM. The calibration curve was linear over the concentration range of 0.5-10 µg/mL and analytical recovery was more than 95%. The coefficients of variation for intra-day and inter-day assays were less than 5%.
Keywords
Nimodipine; Nimodipine oxide; HPLC; Cerebrospinal fluid; Polymeric micelle
Main Subjects
Medicinal chemistry
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