Teratogenic Effects of Intravitreal Injection of Bevacizumab in a Pregnant Rat Model

Document Type: Research article

Authors

1 Residence of Ophthalmology, Department of ophthalmology, Shiraz University of Medical Sciences, Shiraz, Iran

2 Assistand Professor of ophthalmology, Department of ophthalmology, Shiraz University of Medical Sciences, Shiraz, Iran

3 Master of Science in developmental Biology, Department of Developmental Biology, Islamic Azad University, Fars, Iran

4 Pathologist, Department of Pathology, Dr Daneshbod Laboratory, Shiraz, Iran

Abstract

Abstract:
Title: Teratogenic Effects of Intravitreal Injection of Bevacizumab in Pregnant Rat Models
Aim: To investigate teratogenic effect of intravitreal injection of bevacizumab in pregnant rat model
Methods: Twenty seven female Wistar rat were inseminated. Pregnant rats were divided into 6 groups (three groups as case and three as control groups). Each case and control groups divided according to the day of intravitreal injections (day 2, 10 and 18). Rats in the case groups received 4 µl intravitreal injection of bevacizumab and the control groups received same volume of distilled water.
Results: The tail and umbilical cord length in case groups 1, 2 and 3 did not display any significant differences compared with their control groups. The fetal weight was significantly lower in the case groups 1 (p<0.001) and 2 (p<0.001) compared with their control groups. Furthermore the placental weight was only lower in case group 1 (p<0.001). Case group 2 had a shorter crown rump length in comparison with its control group (p=0.029). Morphological investigations showed two abnormal cases of gastroschisis in group 1 and a case of a cleft in the skull in one of the rats in case group 2.
Conclusion: Intravitreal bevacizumab has developmental effect when administered in the early stages of pregnancy; but it is safe when administered in the last week of pregnancy in rats.

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