Characterization of Miconazole effects on Mice fetus liver tissue using FTIR-MSP

Document Type: Research article

Authors

1 Occupational Health Research Center and Department of Occupational health, Faculty of public health, University of Iran/ Medical Sciences, Tehran, Iran

2 Young Researchers and Elite Club, Central Tehran Branch, Islamic Azad University,Tehran, Iran.

3 Department of Pharmacology/Toxicology, Faculty of Pharmacy, University of ShahidBeheshti/ Medical Sciences, Tehran, Iran

Abstract

Azole agents especially Miconazole are widely used even during pregnancy as antifungal agents. The aim of this study was to assess the usefulness of FTIR Micro-Spectroscopy for discriminating of Miconazole treated liver tissue from control liver tissue. The mice were injected with Miconazole (60 mg/Kg) on gestation day 9 and they were dissected on pregnancy day 15. The fetus fixed, dehydrated and embedded in paraffin. Sections of liver (10 μm) were prepared from control and treated fetus groups by Microtome and deparaffinized with xylene. The spectra were collected using FTIR-MSP in the region of 4000-400 cm-1. All spectra were normalized to amide II band (1454cm-1) after baseline correction of entire spectrum. The results were shown by 2nd derivatization of spectra and also subtracting from control spectra. Miconazole induces some minor changes in the mouse fetus liver at cellular levels when mother is exposed. The most important calculated alterations are in the production of fetus liver proteins. α helical and β sheet structures have shown significant variations, indicating protein alterations configurationally

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