Green Synthesis of Silver Nanoparticles using Bidens Frondosa Extract and their Tyrosinase Activity

Document Type: Research article


1 Department of Biology Kongju national University Korea

2 Department of Chemistry, Kongju national University Korea

3 Department of Biology Kongju National university korea

4 Department of Biology Kongju national university Korea

5 Department of Biology, Kongju National University

6 Department of Chemistry, Kongju National University Korea

7 Professor


Herbal nanoparticles gain lot of attention because of their pharmaceutical importance. The present study reports the eco-friendly synthesis, characterization and their tyrosinase activity of silver nanoparticles (AgNPs) using aqueous extract of Bidens frondosa. The appearance of brown color indicated the formation of B. frondosa AgNPs. The Formation of AgNPs was confirmed by UV–Vis spectroscopy, FTIR, FESEM and EDS analysis. The formation of herbal AgNPs of size ranging 20-70 nm further were assured by energy dispersive X-ray spectroscopy (EDS) and field emission scanning electron microscopy (FESEM).The mushroom tyrosinase inhibitory activity of synthesized AgNPs was evaluated. Nanoparticles were found to have significant higher tyrosinase inhibitory activity compared to control. The IC50 values of crude extract, AgNP and Kojic acid were found to be 9, 15, and 2.37 μg/mL, respectively. AgNPs of B. frondosa may be considered as potential candidate for the production of medical and cosmetic products.


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