rabiei, M., Mehdizadeh, M., Rastegar, H., Vahidi, H., Alebouyeh, M. (2013). Detection of genetically modified maize in processed foods sold commercially in Iran by qualitative PCR. Iranian Journal of Pharmaceutical Research, 12(1), 25-30. doi: 10.22037/ijpr.2013.2012
maryam rabiei; Mehrangiz Mehdizadeh; Hossein Rastegar; Hossein Vahidi; Mahmoud Alebouyeh. "Detection of genetically modified maize in processed foods sold commercially in Iran by qualitative PCR". Iranian Journal of Pharmaceutical Research, 12, 1, 2013, 25-30. doi: 10.22037/ijpr.2013.2012
rabiei, M., Mehdizadeh, M., Rastegar, H., Vahidi, H., Alebouyeh, M. (2013). 'Detection of genetically modified maize in processed foods sold commercially in Iran by qualitative PCR', Iranian Journal of Pharmaceutical Research, 12(1), pp. 25-30. doi: 10.22037/ijpr.2013.2012
rabiei, M., Mehdizadeh, M., Rastegar, H., Vahidi, H., Alebouyeh, M. Detection of genetically modified maize in processed foods sold commercially in Iran by qualitative PCR. Iranian Journal of Pharmaceutical Research, 2013; 12(1): 25-30. doi: 10.22037/ijpr.2013.2012
Detection of genetically modified maize in processed foods sold commercially in Iran by qualitative PCR
1Food and Drug Laboratory Research Center,Food and Drug Organization, MOH and ME
2Food and drug control reference laboratory, Food and Drug Organization, MOH and ME
3Food and drug laboratory research center, Food and Drug Organization, MOH and ME
4Faculty of pharmacy, Shaheed Beheshti university of medical sciences and health services
5Food and drug laboratory research center, Food and Drug Organization,MOH and ME
Abstract
Detection of genetically modified organisms (GMOs) in food is an important issue for all the subjects involved in food control and customer’s right. Due to the increasing number of GMOs imported to Iran during the past few years, it has become necessary to screen the products in order to determine the identity of the consumed daily foodstuffs. In this study, following extraction of genomic DNA from processed foods sold commercially in Iran, qualitative PCR was performed to detect genetically modified maize. The recombinant DNA target sequences were detected with primers highly specific for each investigated transgene such as CaMV35s gene, Bt-11, MON810 and Bt-176 separately. Based on the gel electrophoresis results, Bt-11 and MON810 events were detected in some maize samples; while in none of them Bt-176 modified gene was detected. The results demonstrate for the first time the presence of genetically modified maize in Iranian food products, reinforcing the need for the development of labeling system and valid quantitative methods in routine analyses.
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