Hepatoprotective effect of Acantholimon gilliati Turril, kew.Bull. on formaldehyde induced liver injury in adult male mice.

Document Type : Research article

Authors

1 Guilan university of medical sciences , School of Pharmacy,Pharmacognosy Department. Research and development center of plants and medicinal chemistry, Guilan University of Medical Sciences, Rasht, Iran.

2 Department of Anatomical Sciences, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran.

3 Student Research Center, Guilan University of Medical Sciences, Rasht, Iran

4 Department of social Medicine, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran.

Abstract

Background: Acantholimon gilliati Turril(Plumbaginaceae) is used in variety of diseases including hepatic ailments in the west region of Iran. Aim of the study: To investigate the hepatoprotective effect of methanoilc extract (ME) of A. gilliati on formaldehyde (FA) induced liver injury in adult male mice.
Materials and methods: Fifty six adult male mice were divided into 8 groups. In control group received normal saline. Group II treated with formaldehyde 10mg/kg. Group III to VIII treated with both FA (10mg/kg) and metalonic extract of plant at doses of 5, 10, 15, 20, 50 and 100 mg respectively. All animals in 8 group were treated for 2 weeks (once every other day). At the end of experiment evaluations were made especially by morphology and photometric method for assay of histopathology of liver and serum levels of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP).
Results: Formaldehyde induced liver damage both in histology and function. The levels of ALT, AST and ALP enzymes were significantly increased in FA treated group. Administration of ME in all experimental groups significantly reduced serum levels of ALT and ALP (P= 0.02). however AST was reduced significantly just in groups III and IV(p<0.05) who were treating with doses of 5 and 10 mg of A. gilliati similarly ME in doses of 5 and 10 mg protected liver histology against FA.
Conclusion: Results showed that the ME of the A. gilliati in the lower doses has a protective effect on both histology and function of liver.

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Iranian Journal of Pharmaceutical Research
Volume 16, Special Issue
Winter 2017
Pages 135-141

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