afzali, M., baharara, J., shahrokhabadi, K., amini, E. (2017). Evaluation of the cytotoxic effect of the Brittle Star (Ophiocomaerinaceus) dichloromethane extract and doxorubicin on EL4 cell line. Iranian Journal of Pharmaceutical Research, 16(Special Issue), 216-226. doi: 10.22037/ijpr.2017.1983
mahbobeh afzali; javad baharara; khadijeh shahrokhabadi; elah amini. "Evaluation of the cytotoxic effect of the Brittle Star (Ophiocomaerinaceus) dichloromethane extract and doxorubicin on EL4 cell line". Iranian Journal of Pharmaceutical Research, 16, Special Issue, 2017, 216-226. doi: 10.22037/ijpr.2017.1983
afzali, M., baharara, J., shahrokhabadi, K., amini, E. (2017). 'Evaluation of the cytotoxic effect of the Brittle Star (Ophiocomaerinaceus) dichloromethane extract and doxorubicin on EL4 cell line', Iranian Journal of Pharmaceutical Research, 16(Special Issue), pp. 216-226. doi: 10.22037/ijpr.2017.1983
afzali, M., baharara, J., shahrokhabadi, K., amini, E. Evaluation of the cytotoxic effect of the Brittle Star (Ophiocomaerinaceus) dichloromethane extract and doxorubicin on EL4 cell line. Iranian Journal of Pharmaceutical Research, 2017; 16(Special Issue): 216-226. doi: 10.22037/ijpr.2017.1983
Evaluation of the cytotoxic effect of the Brittle Star (Ophiocomaerinaceus) dichloromethane extract and doxorubicin on EL4 cell line
21. Animal Developmental Biology, Research center for Animal Development Applied Biology& Dept. Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran
3mashhad Branch,Islamic azad University
4kharazmi university
Abstract
Leukemia is a blood disease which is caused by the inhibition of differentiation and an increased proliferation rate. Nature has been known as a rich source of medically useful substances. High diversity of bioactive molecules extracted from marine invertebrates, makes them ideal candidates for cancer researches. The study has been done to investigate cytotoxic effects of dichloromethane brittle star extract and doxorubicin on EL4 cancer cells. Blood cancer EL4 cells were cultured and treated by different concentrations of brittle star dichloromethane extract for 24, 48 and 72 h. Cell toxicity was studied using the MTT assay. Cell morphology was examined using an invert microscope. Further, apoptosis was examined using Annexin V-FITC, propodium iodide, DAPI and Acridine orange / propodium iodide staining. Eventually, the apoptosis pathways were analyzed using measurement of caspase-3 and -9 activities. The statistical analysis was performed using SPSS software, ANOVA test, Tukey post test. p
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