1Guilan university of medical sciences , School of Pharmacy,Pharmacognosy Department
2Faculty of Pharmacy and Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
3Faculty of Basic sciences , Central Tehran Branch, Islamic Azad University, Tehran, Iran
4Phytochemistry Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran Department of Pharmacognosy, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Isolated five known iridoids glycosides (Scrophuloside A, Harpagoside B, 5-hydroxyloganin, 8-O-acetylharpagide and 6-O-methyl,1-glucopyranosyl catalpol ),one phenyl ethanoid glycoside (Verbascoside) and a phenyl ethanol amine (2-(4-Chlorobenzyl amino) ethanol ) compound from the methanolic extract of aerial parts of Scrophularia oxysepala using by high performance liquid chromatography based on isocratic and liner gradients by C18 column . The structure elucidations of the isolated compounds were performed by spectroscopic methods include 1H-NMR, 13C-NMR, 2 D NMR technique such as HMBC ( in deuterated methanol as solvent) , GC-MS and UV also methanolic extract and fractions ( fractionated on solid phase extraction on C18 cartridge(Spack-C18)) of this plant was tested for free radical scavenging properties toward the 1, 1-diphenyl-1-picrylhydrazyl (DPPH), general toxicity (Brine shrimp toxicity assay) , insecticidal ( Contact toxicity insecticidal assay) and antimalarial activities (hemebiocrystallization inhibition assay).