Chitin from the mollusc Chiton: Extraction, characterization and chitosan preparation

Document Type: Research article

Authors

1 PhD student, Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Professor, Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

3 Professor, Research Center for Animal Development, Applied Biology & Biology Department, Mashhad Branch, Islamic Azad University, Mashhad, Iran

4 Professor, Biotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

5 Assistant professor, Institute of Tropical Forestry and Forest Products (INTROP), University Putra Malaysia, UPM Serdang, Selangor 43400, Malaysia

Abstract

This study presents the first ever data of extracting chitin from the Chiton shell, which then converted to the soluble chitosan by soaking in the 45% NaOH solution. The obtained chitin and chitosan were characterized by the seven different methods. Antioxidant activity of the extracted chitosan was also evaluated using the two methods. The shell content was divided into calcium carbonate (90.5 %), protein (5.2%) and chitin (4.3 %). Due to the results of element analysis and 1H NMR, the final degree of deacetylation of chitosan was 90%. Surprisingly, a significant amount of Fe was accidentally found in the shell after demineralization, and removed from the solution through the filtering. Nonetheless, remained Fe in the extracted chitin and chitosan was 20 times higher than those previously reported from the shell of shrimps and crabs. Presence of this amount of Fe could describe that why the produced chitosan was darker compared to the commercial chitosan. Antioxidant activity tests showed that the IC50 of the extracted chitosan was higher than which estimated for the commercial chitosan. Antioxidant activity of the extracted chitosan is even better than the commercial version and may use in pharmaceutical industry as a source of antioxidant.

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