Antioxidative effect of aqueous Date fruit extract in PC12 cell line



Cell damage induced by free radicals has been implicated as causal factor in a wide variety of human diseases such as cancer, neurodegenerative disorders. Scientific recognition of natural antioxidants may be helpful in preventing many diseases. Aqueous Date fruit extract (ADFE)- PC12 cell line. ADFE at concentrations of 0.1-10% containing 0.005% H2O2 and H2O2 at concentrations of 0.01-1% containing 10% ADFE were prepared. 100µl from a cell suspension equal to 106 cells/ml of PC12 cells were added to centrifuge tubes and incubated for 24 hours. Cell suspension rinsed twice with medium and incubated in 96-multidish for 72 hrs. Then, cell growth was assayed by XTT-assay. Aqueous Date fruit extract (ADFE) at concentrations of 0.1, 1 and 10% comparing to 0.005 H2O2 showed significant increase in PC12 cell growth (by 11, 57 and 74% respectively) (P<0.05 to 0.001). ADFE at concentration of 10% showed protective effects against different concentrations of H2O2 (0.01-1%)(P<0.05 to P<0.001). Date fruit contains compounds with potent antioxidant activity and daily consumption may be helpful in preventing many diseases, especially neurodegenerative disorders such as Alzheimer’s disease and Parkinson’s disease.