Doxorubicin Loaded DNA Aptamer Linked Myristilated Chitosan Nanogel for Targeted Drug Delivery to Prostate Cancer

Document Type : Research article

Authors

1 Associate of prod.,Departmaent of Genetics,IAU,Tehran medical branch

2 Department of Biology, Science and research branch, Islamic Azad University, Tehran, IRAN

3 Department of Biology, Faculty of Science, Shahed University, Tehran, IRAN

Abstract

Recently, specific attention has been paid to aptamers, short DNA or RNA, as a tool for cancer diagnosis and therapy. In the present study MCS nanogels were prepared by Myristate: chitosan at 1:9 ratio and were characterized by several techniques. A selected ssDNA aptamer(Apt) capable of detecting LNCaP cells was linked to Myristilated chitosan nanogels (Apt-MCS) by glutaraldehyde and loaded with Doxorubicin (DOX) to be used in targeted drug delivery against the Prostate cancer cells. LNCaP and PC-3 cells were treated with Apt-MCS-DOX complex and the binding efficiency was estimated by flow cytometry. The binding affinity of the selected aptamers was above 70% compared to the initial library. The loading capacity of the nanogel was as high as 97% and up to 40% of DOX were released from MCS within 15 days.Cytotoxicity of nanodrugs on LNCaP cells were determined by MTT assay. Apt-MCS-DOX was specifically binded to LNCaP cells whereas it didn’t show any specificity to PC-3 cells as a negative control. Both MCS-DOX and Apt-MCS-DOX showed a lethal effect on LNCaP cells. Our results can lead to an aptamer based simple and applicable technique for early diagnosis and treatment of cancerous cells.
Keywords: DOX; MCS; nanogel; aptamer; Targeted drug delivery; Prostate cancer.

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Iranian Journal of Pharmaceutical Research
Volume 16, Issue 1
Winter 2017
Pages 35-49

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