PREPARATION AND OPTIMISATIONOF PALM-BASED LIPID NANOPARTICLES LOADED WITH GRISEOFULVIN

Document Type: Research article

Authors

1 Malaysia Palm Oil Board (MPOB) 6, Jalan Institusi, 43000 Kajang, Selangor DE

2 Division of Pharmaceutical Chemistry, School of Pharmacy and Health Sciences, International Medical University, No. 126, Jalan Perkasa 19, Bukit Jalil, 57000 Kuala Lumpur

3 Division of Pathology, Faculty of Medicine and Health & School of Postgraduate Studies and Research, International Medical University, No. 126, Jalan Perkasa 19, Bukit Jalil, 57000 Kuala Lumpur

Abstract

Palm-based lipid nanoparticle formulation loaded with griseofulvin was prepared by hot homogenisation and a solvent-free method. The optimum griseofulvin loaded lipid nanoparticles were prepared via stages of optimisation, such as high pressure homogenisation (HPH) parameters, lipid and surfactant screening and selection of suitable lipid to surfactant ratios; followed by photon correlation study. A HPLC method has also been validated for the drug loading capacity study. The optimum HPH parameter was determined to be 1500 bar with 5 cycles and among the lipid based materials; Lipid C (triglycerides) was selected for the preparation of lipid nanoparticles. Tween 80 was chosenfrom the Tween series surfactantsfor its highest saturated solubility of griseofulvinat 53.1 ± 2.16 µg/mL. The optimum formulation of the griseofulvin loaded lipid demonstrated nano range particle size (179.8 nm) withintermediate distribution index (PDI) of 0.306, zeta potential of -27.9 mV and drug loading of 0.77%. The formulation was stable upon storage for 1 month at room temperature (25ºC) and higher temperature (45ºC) with consistent drug loading capacity.

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