2Neurogenic Inflammation Research Centre and Dept. of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
3Pharmaciutical Research Center and Dept. of Physiology School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
4Student Research Committee, Faculty of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
Abstract
The stimulatory effect of the extract of Portulaca oleracea (P. olerace) on β-adrenoceptor of tracheal smooth muscle was examined. To examine β-adrenoceptor stimulatory effect, concentration response curve to isoprenaline was obtained in pre-contracted tracheal smooth muscle in the presence of three concentrations of aqueous-ethanolic extract, propranolol and saline. Values of EC50 (the effective concentration of isoprenaline, causing 50% of maximum response) and dose ratio -1 (CR-1) were measured. This effect was tested in non-incubated tracheal smooth muscle (group 1) and incubated tissues with chlorpheniramine (group 2). Concentration-response curves to isoprenaline in the presence of two higher concentrations of the extract in group 1 and all three concentrations in group 2 showed leftward shifts compared to isoprenaline curves produced in the presence of saline in both groups. EC50 obtained in the presence of propranolol was significantly higher than that of saline in both groups of experiments (p
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