Evaluation of In Vitro Antimalarial Activity of Different Extracts of Eremostachys azerbaijanica Rech.f.

Document Type: Research article

Authors

1 Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

2 Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

3 Drug Applied research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

4 Drug Applied Research Center, Tabriz University of Medical Scineces, Tabriz, Iran.

Abstract

Six extracts with different polarity from aerial parts and rhizomes of Eremostachys azerbaijanica Rech.f., were screened for their antimalarial properties by cell free 𝛽-hematin formation assay. Dichloromethane (DCM) extracts of both parts of plant showed significant antimalarial activities with IC50 values of 0.949 ± 0.061 mg/mL in aerial parts and 0.382 ± 0.011 mg/mL in rhizomes. Bioactivity-guided fractionation of the most potent part (DCM extract of rhizomes) by vacuum liquid chromatography (VLC) afforded seven fractions. Two fractions (100% Ethyl acetate (EtOAC) and 100% Methatol (MeOH)) showed considerable antimalarial activity with IC50 values of 0.335 ± 0.033 mg/mL and 0.403 ± 0.037 mg/mL, respectively.
According to GC-MS analysis, sesquiterpenes, steroids and coumarines derivatives are the main constituents of the most potent fractions; therefore, it seems that the anti malarial activity of these fractions may be related to the presence of these types of compounds.

Keywords

Main Subjects