Simultaneous Chloramphenicol and Florfenicol Determination by A Validated DLLME-HPLC-UV Method in Pasteurized Milk

Document Type: Research article

Authors

1 Medicinal and Natural Products Chemistry Research Center, Shiraz University of Medical Sciences, P.O. Box 71345-3288, Shiraz, Iran

2 Medicinal and Natural Products Chemistry Research Center, Shiraz University of Medical Sciences, P.O. Box: 71345-3388, Shiraz, Iran

3 Department of Medicinal Chemistry, School of Pharmacy, Shahid Beheshti Medical University, P.O. Box 14155-6153, Tehran,Iran

Abstract

The antibiotic residues in milk are a well-known serious problem and pose several health hazards to consumers. We have described a simple, rapid, and inexpensive DLLME-HPLC/UV technique for the extraction of chloramphenicol and florfenicol residues in milk samples. Under the optimum conditions, linearity of the method was observed over the range 0.02-0.85 µg/L with correlation coefficients > 0.999. The proposed method has been found to have a good limit of detection (signal to noise ratio = 3) for chloramphenicol (12.5 µg/kg) and florfenicol (12.2 µg/kg), and precision with relative standard deviation values under 15% (RSD, n = 3). Good recoveries (69.1–79.4 %) were obtained for the extraction of the target analytes in milk samples. This simple and economic method has been applied for analyses of 15 real milk samples. Among all samples only one of them was contaminated to florfenicol; 62.4 µg/kg and contamination to chloramphenicol was not detected.

Keywords

Main Subjects