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Iranian Journal of Pharmaceutical Research
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Kazeem, M., Mayaki, A., Ogungbe, B., Ojekale, A. (2016). In-vitro Studies on Calotropis procera Leaf Extracts as Inhibitors of Key Enzymes Linked to Diabetes Mellitus. Iranian Journal of Pharmaceutical Research, 15(Special Issue), 37-44. doi: 10.22037/ijpr.2016.1804
Mutiu Idowu Kazeem; Ayuva Mercy Mayaki; Bimpe Folashade Ogungbe; Anthony Babajide Ojekale. "In-vitro Studies on Calotropis procera Leaf Extracts as Inhibitors of Key Enzymes Linked to Diabetes Mellitus". Iranian Journal of Pharmaceutical Research, 15, Special Issue, 2016, 37-44. doi: 10.22037/ijpr.2016.1804
Kazeem, M., Mayaki, A., Ogungbe, B., Ojekale, A. (2016). 'In-vitro Studies on Calotropis procera Leaf Extracts as Inhibitors of Key Enzymes Linked to Diabetes Mellitus', Iranian Journal of Pharmaceutical Research, 15(Special Issue), pp. 37-44. doi: 10.22037/ijpr.2016.1804
Kazeem, M., Mayaki, A., Ogungbe, B., Ojekale, A. In-vitro Studies on Calotropis procera Leaf Extracts as Inhibitors of Key Enzymes Linked to Diabetes Mellitus. Iranian Journal of Pharmaceutical Research, 2016; 15(Special Issue): 37-44. doi: 10.22037/ijpr.2016.1804

In-vitro Studies on Calotropis procera Leaf Extracts as Inhibitors of Key Enzymes Linked to Diabetes Mellitus

Article 5, Volume 15, Special Issue, Winter 2016, Page 37-44  XML PDF (809.6 K)
Document Type: Research article
DOI: 10.22037/ijpr.2016.1804
Authors
Mutiu Idowu Kazeem email 1; Ayuva Mercy Mayaki2; Bimpe Folashade Ogungbe2; Anthony Babajide Ojekale2
1Department of Biochemistry, Lagos State University, Ojo, Lagos, Nigeria.
2Department of Biochemistry, Lagos State University, PMB 0001, Ojo, Lagos, Nigeria.
Abstract
The side effects associated with the usage of synthetic antidiabetic drugs make it imperative to search for alternative drugs from medicinal plants. Therefore, this study was aimed at evaluating the α-amylase and α-glucosidase inhibitory potential of Calotropis procera leaf, as well as its possible mode of inhibiting these enzymes. Acetone, aqueous and ethanolic extracts of C. procera leaf was subjected to standard enzymes’ inhibitory assay in-vitro using porcine pancreatic α-amylase and rat intestinal α-glucosidase. Results obtained showed that out of all the extracts tested, ethanolic and aqueous extracts possessed the best inhibition of α-amylase (IC50 7.80 mg/mL) and α-glucosidase (3.25 mg/mL) respectively. The kinetic analysis of the mode of inhibition of these enzymes by the leaf extracts of C. procera, revealed that these extracts inhibited both enzymes in a non-competitive manner. It is speculated that the α-amylase and α-glucosidase inhibitory properties of leaf extracts of C. procera may be due to the presence of some phytochemicals such as flavonoids, tannins and saponins in the plant. It can be concluded from this study that the Calotropis procera extracts could serve as source of antidiabetic agents which may act through the inhibition of carbohydrate hydrolyzing enzymes, α-amylase and α-glucosidase.
Keywords
Calotropis procera; Diabetes mellitus; α-amylase; α-glucosidase; tannins
Main Subjects
Pharmacognosy
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